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Vitamin deficiencies usually develop slowly over several months to years. Vitamin deficiency symptoms may be subtle at first, but they increase as the deficiency worsens. Vitamins may be fat soluble (vitamins A, D, E, and K) or water soluble (B vitamins and vitamin C). The B vitamins include biotin (vitamin B7), folate, niacin, pantothenic acid, riboflavin (B2), thiamin (B1), B6 (e.g., pyridoxine), and B12 (cobalamins). If symptoms such as hair loss, brittle nails, skin changes, or suspected nutritional deficiencies are present, your doctor may recommend a Biotin Test to assess biotin (vitamin B7) levels as part of the evaluation. Vitamin C, another water-soluble vitamin, plays an important role in collagen formation, wound healing, immune function, and antioxidant protection. If vitamin C deficiency is suspected, your doctor may recommend a Vitamin C Test to evaluate vitamin C levels as part of the nutritional assessment. Who is at risk of Vitamin Deficiency? Pregnant women Children Diabetics Elderly Thyroid patients Patients recovering from an infection like Dengue or Malaria Patients with tuberculosis Patients who have had a recent surgery Chronic illness leads to vitamin deficiency