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Vitamin D Test

Also known as: Vitamin D Test (25-Hydroxy)

78+ booked in last 3 days

Vitamin D Test (25-Hydroxy) Details in Brief

Also Known As Vitamin D Total - 25 Hydroxy (OH), 25-Hydroxyvitamin D Test, Calcifediol Test
Purpose Measures total 25-hydroxy vitamin D levels, helps assess bone health and calcium metabolism, supports the diagnosis of vitamin D deficiency, and aids in monitoring osteoporosis treatment.
Preparation A high biotin dose (>5 mg/day) should be avoided at least 8 hours before blood collection.
Fasting Not Required
Reporting Time After 6 hours
Cost 1,800

What Is a Vitamin D Test?

A vitamin D test is a blood test that measures the amount of vitamin D in your body. This simple test helps your doctor determine if you have vitamin D deficiency or, less commonly, vitamin D toxicity, both of which can affect your bone health and overall well-being.

What Does the Vitamin D Test Measure?

The test specifically measures your body's vitamin D status through the following:

25-Hydroxyvitamin D levels: This is the main form of vitamin D circulating in your blood and provides the most accurate picture of your vitamin D status.
Total vitamin D concentration: Shows whether your levels fall within deficient, insufficient, sufficient, or toxic ranges.
Vitamin D reserves: Reflects the vitamin D your body produces from sunlight exposure, as well as what you get from food and supplements.
Calcium metabolism indicators: Helps assess how well your body can absorb calcium for strong bones.

Vitamin D Test: Who Needs It and Why It's Done

What Symptoms May Call for a Vitamin D Test?

A doctor may recommend a vitamin D test if you are experiencing symptoms of a deficiency, which can include:

• Bone pain and joint pain, especially in your back
• Frequent illness or infections due to a weakened immune system
• Persistent fatigue and feeling exhausted without a clear reason
• Muscle weakness, aches, or unexpected muscle cramps
• Mood changes, including feelings of depression
• Unexplained hair loss
• Slow wound healing after injuries or surgery

Who Should Get a Vitamin D Test?

Several groups of people should consider getting tested for vitamin D levels:

People experiencing symptoms: Anyone with the symptoms mentioned above
Older adults: As people age, their skin's ability to produce vitamin D from sunlight decreases.
People with limited sun exposure: Those who spend most time indoors, live in highly polluted cities, or wear clothing covering most skin.
People with darker skin tones: Higher amounts of melanin reduce the body's ability to produce vitamin D from sunlight.
People with obesity: Vitamin D can become trapped in fat tissue, making it less available to the body.
Pregnant and breastfeeding women: They have increased vitamin D requirements.
Patients with certain medical conditions: People with conditions affecting fat absorption or chronic kidney/liver disease.
People on specific medications: Such as steroids or anti-seizure medicines that can interfere with vitamin D.

Why Is a Vitamin D Test Done?

The vitamin D test provides an accurate diagnosis of vitamin D deficiency or toxicity, allowing doctors to prescribe the correct supplement dosage and prevent serious complications like osteoporosis and bone fractures.

Importance of a Vitamin D Test

The vitamin D test plays a crucial role in your healthcare, as it is essential for calcium absorption and bone health. Without adequate vitamin D, your body cannot properly absorb calcium, leading to weakened bones and increased fracture risk. This test is clinically important for accurate diagnosis of vitamin D status, guiding appropriate treatment with supplements, and monitoring your response to therapy.

For high-risk individuals, it serves as a preventive tool to identify deficiencies before serious bone problems develop. The test results help your doctor determine the right dosage of vitamin D supplements, as both deficiency and excess can be harmful to your health.

Vitamin D Test Booking & Reports – Metropolis Healthcare India

How to Book a Vitamin D Test?

  1. Simple Online Booking
    Booking can be done through the Metropolis Healthcare App or website. Select the 'Vitamin D Test', choose a convenient time slot, and provide your address for a blood test at home. You can also visit the nearest Metropolis Lab if you prefer to give the sample directly at the lab.
  2. Safe Home Sample Collection
    Our trained phlebotomists ensure the timely collection of your sample while strictly following all safety and hygiene protocols.
  3. Sample Tracking Updates
    Stay informed at every step. From collection to testing, you can track your sample directly through the Metropolis Healthcare website.
  4. Accurate Laboratory Testing
    Your sample is processed at our NABL & CAP accredited laboratories, where expert technicians ensure accurate results.
  5. Quick & Easy Reports
    Receive your test reports promptly via email, WhatsApp, or by downloading them directly from the Metropolis Healthcare website or app.

Is Home Sample Collection for the Vitamin D Test Available Near You?

Yes, Metropolis Healthcare provides home sample collection for the vitamin D 25-hydroxy test. Our trained phlebotomists follow strict safety protocols while collecting your blood sample in the comfort of your home. This convenient service eliminates travel time and waiting queues, making it easier for you to prioritise your health.

How Long Does It Take to Get a Vitamin D Test Report?

Reports are usually available within 6 hours once the sample reaches the lab.

Where Can I See or Get Vitamin D Test Results?

Test results from Metropolis Healthcare can be accessed through multiple convenient channels. You can log in to the Metropolis website using your credentials or use the Metropolis Healthcare App to view and download your reports. Additionally, test reports are sent via email or WhatsApp, and you also have the option to collect a physical copy directly from the lab.

Interpreting Vitamin D Test Results

What Your Vitamin D Test Results May Indicate

The table below shows the different ranges for vitamin D levels and what they mean for your health:

Parameter

Reference Range

Low Values Indicate

High Values Indicate

Vitamin D Total - 25 Hydroxy (OH)

Deficiency: < 20 ng/mL


Insufficiency: 20-30 ng/mL


Sufficiency: 30-100 ng/mL


Hypervitaminosis: > 100 ng/mL

Deficiency or insufficiency may lead to bone problems, muscle weakness, increased infection risk, and calcium absorption issues.

Hypervitaminosis (toxicity) can cause nausea, vomiting, kidney problems, and excessive calcium absorption.

 

Conditions that May Affect Vitamin D Test Accuracy

Several factors can influence your vitamin D test results and should be discussed with your doctor:

High biotin supplements: Doses greater than 5 mg per day can interfere with test results, requiring an 8-hour gap before blood collection.
Seasonal variations: Winter months may show lower levels due to reduced sun exposure.
Liver or kidney disease: These conditions can affect vitamin D metabolism and conversion.
Malabsorption disorders: Conditions like Crohn's disease or coeliac disease can impact vitamin D absorption.
Medications: Certain medications, including steroids and anti-seizure medications, can lower vitamin D levels.
Body fat percentage: Higher body fat can affect vitamin D availability in your bloodstream.
Recent illness or stress: These can temporarily affect your vitamin D metabolism.

How Is a Vitamin D Test Done?

The vitamin D test involves a simple blood collection procedure:

• A healthcare professional will clean the area around a vein in your arm with an antiseptic.
• A tourniquet is applied to your upper arm to make the veins more visible.
• A needle is inserted into the vein to collect approximately 3 ml of blood in a gel vacutainer.
• The tourniquet is removed, and pressure is applied to the puncture site.
• Your blood sample is labelled and sent to the laboratory for analysis using the electrochemiluminescence immunoassay (ECLIA) method.
• The entire procedure takes just a few minutes and causes minimal discomfort.

How Should You Prepare for a Vitamin D Test?

Preparing for your vitamin D test is straightforward with minimal requirements:

No fasting required: You can eat and drink normally before the test.
Avoid high biotin supplements: Stop taking biotin supplements exceeding 5 mg per day for at least 8 hours before blood collection.
Inform about medications: Tell your doctor about any medications or supplements you're taking.
Wear comfortable clothing: Choose clothes with sleeves that can be easily rolled up for blood collection.
Stay hydrated: Drink adequate water before the test to make blood collection easier.

Diseases that a Vitamin D Test Can Help Detect

The vitamin D test can help identify or monitor several health conditions related to vitamin D deficiency or excess:

Osteoporosis: Weakening of bones due to poor calcium absorption
Rickets: Bone deformities in children caused by severe vitamin D deficiency
Osteomalacia: Softening of bones in adults
Secondary hyperparathyroidism: Overactive parathyroid glands compensating for low vitamin D
Muscle weakness disorders: Conditions causing unexplained muscle pain and weakness
Immune system disorders: Frequent infections or autoimmune conditions
Vitamin D toxicity: A rare condition from excessive vitamin D supplementation

Vitamin D Tests/Packages that You Can Book With Metropolis Healthcare

The following related tests and packages are available at Metropolis Healthcare to provide a comprehensive assessment of your vitamin D status and bone health:

Test/Package Name

Purpose/Highlights

Vitamin D-1,25-Dihydroxy Serum

Measures the active form of vitamin D for detailed assessment

Vitamin D Plus Profile

Comprehensive vitamin D assessment with additional parameters

Vitamin D Profile - Gold (Vitamin D2, Vitamin D3 & D Total) Test

An advanced test measuring different forms of vitamin D separately

 

Vitamin D Test Prices in Different Cities

The cost of a vitamin D test may vary depending on your location. Here's a table showing the approximate prices in different cities:

City

Price (₹)

Mumbai

₹900 to ₹1900

Delhi

₹900 to ₹1900

Bangalore

₹900 to ₹1900

Chennai

₹900 to ₹1900

Pune

₹900 to ₹1900

Ahmedabad

₹900 to ₹1900

Read More

Vitamin D Test (25-Hydroxy) Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Vitamin D Test (25-Hydroxy) with a clear pricing structure.

The Vitamin D Test (25-Hydroxy) Price in Mumbai is ₹ 1,800 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

The recommended daily amount of vitamin D for adults is typically 600 International Units (IU). However, some people may need higher doses as prescribed by a doctor based on their deficiency levels and health conditions.

Vitamin D2 comes from plant sources and fortified foods, while Vitamin D3 is naturally produced in skin from sunlight and found in animal-based foods. Vitamin D3 is generally more effective at maintaining blood levels.

This specialised test is useful for individuals taking vitamin D2 supplements or those with complex deficiency cases. It helps doctors identify the source of vitamin D and monitor treatment effectiveness more precisely.

There's no specific best time of day for the test. Vitamin D levels remain stable in the blood. So you can provide your sample at any convenient time throughout the day.

No fasting is required for a standard vitamin D test. However, if other tests requiring fasting are ordered alongside, follow your doctor's specific instructions for preparation.

Severe dehydration can concentrate blood components, potentially affecting test accuracy. It's best to stay normally hydrated before any blood test to ensure reliable results.

No special dietary preparation is needed. However, inform your doctor about all medications and supplements, especially vitamin D supplements, as they directly affect test results.

A healthcare professional cleans your arm, inserts a small needle into a vein, and collects blood into a vial. The entire process is quick and typically takes less than five minutes.

The normal levels of the vitamin D test are 30-100 ng/mL (sufficiency). Levels below 20 ng/mL indicate deficiency, 20-29 ng/mL show insufficiency, and above 100 ng/mL suggest potential toxicity.

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