Widal Test For Typhoid
Also known as: Widal Test for Typhoid, Serum
Widal Test for Typhoid, Serum Details in Brief
| Also Known As | Typhoid Test, Enteric Fever Test |
|---|---|
| Purpose | Serological test for Salmonella typhi infection; aids in detecting typhoid fever, supports differentiation of enteric fever from other infections |
| Preparation | Clinical History Required |
| Fasting | Not Required |
| Reporting Time | Next Day: 6:00 PM |
| Cost | 560 |
What Is a Widal Test for Typhoid?
The Widal test is a blood test that helps detect typhoid fever by looking for specific antibodies your body produces when fighting typhoid-causing bacteria. It's an agglutination test that measures your immune system's response to Salmonella enterica serotype Typhi, the bacteria responsible for typhoid fever.
The test uses a blood sample taken from a vein in your arm. It helps detect and diagnose typhoid fever, also known as enteric fever, by measuring antibody levels in your blood.
What Does the Widal Test for Typhoid Measure?
The Widal test measures specific antibodies your body creates to fight typhoid infection:
• O antigen antibodies: Target the cell body part of Salmonella bacteria, often indicating an active infection.
• H antigen antibodies: Target the tail-like structures of the bacteria, appearing later in infection and lasting longer.
• Paratyphi A and B antibodies: Help detect related strains that cause similar symptoms.
• Antibody titres: The concentration levels are reported as ratios (1:40, 1:80, 1:160), where higher numbers indicate more antibodies.
Widal Test for Typhoid: Who Needs It and Why It's Done
What Symptoms May Call for a Widal Test for Typhoid?
Doctors may recommend this test if you experience persistent symptoms suggesting typhoid fever:
• Prolonged high fever with step-ladder pattern
• Severe headache and body aches
• Extreme fatigue and weakness
• Stomach pain with constipation or diarrhoea
• Loss of appetite and nausea
• Rose-coloured spots on chest and abdomen (in some cases)
Who Should Get a Widal Test for Typhoid?
This test is particularly important for specific groups of people:
• Symptomatic patients: Individuals with persistent fever and other typhoid symptoms.
• High-risk individuals: People living in or travelling to areas with poor sanitation.
• Endemic region residents: Those in areas where typhoid is common, particularly across India.
• Contact screening: Family members or close contacts of confirmed cases.
• Outbreak surveillance: During community outbreaks for rapid screening.
Why Is a Widal Test for Typhoid Done?
The Widal test helps doctors confirm suspected typhoid fever cases and guide appropriate antibiotic treatment. Early diagnosis prevents serious complications like intestinal bleeding or perforation.
Importance of a Widal Test for Typhoid
The Widal test remains clinically important in India and resource-limited settings because it's widely available, cost-effective, and provides quick results. While blood culture is the gold standard for diagnosis of typhoid, it takes several days for results. The Widal test offers faster guidance for initial antibiotic treatment decisions.
It helps doctors make presumptive diagnoses when combined with clinical symptoms and examination findings. This rapid screening capability is essential for preventing severe typhoid complications and controlling community outbreaks, especially in endemic regions where typhoid remains a significant health concern.
Widal Test for Typhoid Booking & Reports – Metropolis Healthcare India
How to Book a Widal Test for Typhoid?
- Simple Online Booking
Booking can be done through the Metropolis Healthcare App or website. Select 'Widal Test For Typhoid', choose a convenient time slot, and provide your address for a blood test at home. You can also visit the nearest Metropolis Lab if you prefer to give the sample directly at the lab. - Safe Home Sample Collection
Our trained phlebotomists ensure the timely collection of your sample while strictly following all safety and hygiene protocols. - Sample Tracking Updates
Stay informed at every step. From collection to testing, you can track your sample directly through the Metropolis Healthcare website. - Accurate Laboratory Testing
Your sample is processed at our NABL- & CAP-accredited laboratories, where expert technicians ensure accurate results. - Quick & Easy Reports
Receive your test reports promptly via email, WhatsApp, or by downloading them directly from the Metropolis Healthcare website or app.
Is Home Sample Collection for the Widal Test for Typhoid Available Near You?
Yes, home sample collection for the Widal test is widely available across India through Metropolis Healthcare. Our trained phlebotomists visit your home at your preferred time, ensuring safe and hygienic blood collection. This service eliminates travel hassles, especially when you're feeling unwell with fever symptoms.
How Long Does It Take to Get a Widal Test for a Typhoid Report?
Reports are usually available the next day by 6 PM once the sample reaches the lab.
Where Can I See or Get a Widal Test for Typhoid Results?
Test results from Metropolis Healthcare can be accessed through multiple convenient channels. You can log in to the Metropolis website using your credentials or use the Metropolis Healthcare App to view and download your reports. Additionally, test reports are sent via email or WhatsApp, and you also have the option to collect a physical copy directly from the lab.
Interpreting the Widal Test for Typhoid Results
What Your Widal Test for Typhoid Results May Indicate
The following table shows the Widal test normal range for different parameters:
|
Parameter |
Normal Range |
|
Salmonella Typhi 'O' Antigen |
No Agglutination |
|
Salmonella Typhi 'H' Antigen |
No Agglutination |
|
Salmonella Paratyphi A 'H' Antigen |
No Agglutination |
|
Salmonella Paratyphi B 'H' Antigen |
No Agglutination |
Understanding Abnormal Widal Test Results by Parameter
The following table explains what your test results mean and their clinical significance:
|
O antigen Agglutination titre |
H antigen Agglutination titre |
Interpretation |
|
No agglutination |
Low titres < 160 |
Anamnestic reaction/cross-reacting antibodies |
|
Low titres < 80 |
No agglutination |
Anamnestic reaction/cross-reacting antibodies |
|
Low titres < 80 |
Low titres < 160 |
Confirm rise in titres with repeat specimen after 2-3 weeks |
|
>/= 80 |
No agglutination |
Suggestive of enteric fever |
|
No agglutination |
>/= 160, any one of either SH, STA, or STB antigen |
Suggestive of enteric fever |
|
>/= 160 |
>/= 160, any one of either SH, STA, or STB antigen |
Strongly indicates enteric fever |
|
Agglutination present (any titre) |
Agglutination (any titre), more than one of the SH, STA or STB antigen types |
Seen generally post-immunisation |
Conditions that May Affect the Widal Test for Typhoid Accuracy
Several factors can influence your Widal test results and should be discussed with your doctor:
• Previous typhoid vaccination: It may cause false positive results due to vaccine-induced antibodies.
• Past typhoid infection: It can leave residual antibodies that affect current test interpretation.
• Cross-reacting conditions: Such as typhus, malaria, chronic liver disease, and rheumatoid arthritis, may interfere.
• Chronic liver disease (CKD): CKD may interfere with antibody production and test accuracy.
• Timing of the test: Testing too early in the infection may give false-negative results.
• Antibiotic treatment: If antibiotics are taken before testing, they can reduce bacterial load and antibody production.
• Immune system status: Weakened immunity may affect antibody production.
How Is a Widal Test for Typhoid Done?
The blood collection process is simple and follows standard safety protocols:
• A healthcare professional ties an elastic band around your upper arm to make veins visible.
• The collection area (usually inside your elbow) is cleaned with an antiseptic.
• A sterile needle is gently inserted into the vein to draw blood into a collection tube.
• The needle is removed, and a cotton ball or bandage is applied to prevent bleeding.
• Your blood sample is labelled and sent to the laboratory for analysis.
• The entire process takes just a few minutes with minimal discomfort.
How Should You Prepare for a Widal Test for Typhoid?
Preparing for your Widal test is straightforward with minimal requirements:
• No fasting required: You can eat and drink normally before the test.
• Inform your doctor: Discuss all medications you're currently taking, especially antibiotics.
• Mention vaccination history: Tell your doctor if you've received typhoid vaccination.
• Stay hydrated: Drink adequate water before your blood test.
• Wear comfortable clothing: Clothes with sleeves that can be easily rolled up.
• Bring medical history: Carry any previous test reports or medical records.
Diseases That a Widal Test for Typhoid Can Help Detect
The Widal test primarily helps identify bacterial infections of the intestinal tract:
• Typhoid fever: The main infection caused by Salmonella Typhi bacteria
• Paratyphoid fever: Similar infection caused by Salmonella Paratyphi A and B
• Enteric fever: General term for both typhoid and paratyphoid infections
• Salmonella bacteraemia: When bacteria spread through the bloodstream
• Chronic typhoid carrier state: Persistent infection without active symptoms.
Widal Test for Typhoid/Packages that You Can Book With Metropolis Healthcare
Here are comprehensive testing options available for typhoid and fever-related conditions:
|
Test/Package Name |
Purpose/Highlights |
|
Comprehensive screening for typhoid infection with multiple parameters. |
|
|
Rapid detection of recent typhoid infection using IgM antibodies. |
|
|
Complete fever investigation panel, including multiple infectious causes. |
Widal Test for Typhoid Prices in Different Cities
The Widal test prices may vary depending on your location. Here's a table showing the approximate prices in different cities:
|
City |
Price |
|
₹ 200 to ₹ 700 |
|
|
₹ 200 to ₹ 700 |
|
|
₹ 200 to ₹ 700 |
|
|
₹ 200 to ₹ 700 |
|
|
₹ 200 to ₹ 700 |
|
|
₹ 200 to ₹ 700 |
References:
Dutta S, Sur D, Manna B, Sen B, Deb AK, Deen JL, Wain J, von Seidlein L, Ochiai RL, Clemens JD, Bhattacharya JK. Evaluation of a new-generation serologic test for the diagnosis of typhoid fever: data from community - based surveillance in Calcutta, India. Diagnostic Microbiology and Infectious Disease. 2006;56:359–365. doi: 10.1016/j.diagmicrobio.2006.06.024
Widal Test for Typhoid, Serum Price
Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Widal Test for Typhoid, Serum with a clear pricing structure.
The Widal Test for Typhoid, Serum Price in Mumbai is ₹ 560 .
We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.
Frequently Asked Questions
The Widal test is most reliable when performed after the first week of fever. Your body needs 7-10 days to produce detectable antibody levels after infection begins, so testing too early may give false-negative results.
No, fasting is not required for the Widal test. You can eat and drink normally before giving your blood sample unless your doctor advises otherwise for additional tests requiring fasting.
Severe dehydration can concentrate your blood, potentially affecting various test results. However, it's not considered a significant factor that would alter the Widal test diagnostic interpretation.
No special dietary preparation is needed for the Widal test. However, inform your doctor about all medications you're taking, as antibiotics or typhoid vaccination history can influence antibody levels.
A healthcare professional ties an elastic band around your upper arm, cleans the needle site, inserts a sterile needle into your vein, draws blood into a vial, removes the needle, and applies a bandage.
The risks are minimal and similar to any blood draw. You might experience slight pain or bruising at the needle site, and, rarely, dizziness, fainting, or minor infection at the puncture site.
For the O antigen, titres of 1:80 or above can be significant. For the H antigen, titres of 160 or above are considered significant. In healthy individuals, no agglutination is expected for all parameters.
The Widal Test is commonly referred to as the Typhoid Test or Enteric Fever Test, used for diagnosing typhoid and paratyphoid infections.
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We offer a comprehensive range of 4000+ clinical laboratory tests and profiles, which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease.

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