Do you have any queries?

or Call us now at 9982-782-555

back-arrow-image Search Health Packages, Tests & More

Preventive Healthcare

What is Vitamin B12 Test?

Last Updated On: Oct 30 2025

What Is a Vitamin B12 Test?

A vitamin B12 test is a blood test that measures the level of vitamin B12 (Cobalamin) in your body. This essential nutrient supports  red blood cell production, nerve function, and DNA synthesis. A deficiency can lead to serious health issues, making early detection important.

The vitamin B12 blood test price is generally affordable, and the test is a reliable way to assess whether you have an adequate level of this vital nutrient. It can also help diagnose vitamin B12 deficiency, monitor treatment effectiveness, and investigate the underlying causes of certain health conditions.

Why Is a Vitamin B12 Test Done?

Doctors may recommend this test if you:

  • Show signs of fatigue, weakness, or neurological problems.
  • Are over 50, follow a vegetarian/vegan diet, or have absorption issues.
  • Have been diagnosed with anaemia.
  • Are being treated for B12 deficiency and need monitoring.

B12 deficiency can cause various neurological symptoms, such as numbness, tingling, balance problems, and cognitive changes.

Symptoms of Vitamin B12 Deficiency

Vitamin B12 deficiency can develop gradually and present with diverse symptoms, including:

  • Fatigue and weakness
  • Pale or jaundiced skin
  • Shortness of breath
  • Numbness or tingling in hands and feet
  • Difficulty walking or balancing
  • Memory loss or confusion
  • Mood changes (depression or irritability)
  • Inflamed tongue (glossitis), mouth ulcers
  • Loss of appetite and weight loss

How Is the Vitamin B12 Test Performed?

  • A healthcare professional draws a small sample of blood, typically from your arm.
  • No fasting is needed unless instructed.
  • The sample is sent to a lab to analyze your B12 levels.

What Do Vitamin B12 Test Results Mean?

B12 Level (pg/mL) | Interpretation

Below 200

Likely deficient — seek medical advice

200–900

Normal range

Above 900

Could indicate over-supplementation or liver/kidney issues

It's crucial to discuss your vitamin B12 test results with your healthcare provider, who will consider your symptoms, medical history, and other factors to determine the most appropriate course of action. In some cases, additional tests may be needed to confirm the diagnosis or rule out other underlying conditions.

Normal Range of Vitamin B12 Test

The vitamin B12 normal range may vary slightly depending on the laboratory and the specific assay used. However, the typical reference range for serum vitamin B12 is approximately 200 to 900 picograms per millilitre (pg/mL) or 148 to 670 picomoles per litre (pmol/L).

It's important to note that some individuals may experience symptoms of vitamin B12 deficiency even if their levels fall within the lower end of the normal range. In such cases, a thorough clinical evaluation and additional testing may be necessary to determine the appropriate treatment plan.

How to Prepare for a Vitamin B12 Test

Typically, no special preparation is needed for a vitamin B12 test. You can eat and drink normally before the test, and you don't need to fast unless your healthcare provider specifically instructs you to do so. However, it's essential to inform your doctor about any medications or supplements you are taking, as some may affect the accuracy of the test results.

When investigating vitamin B12 deficiency or related conditions, your healthcare provider may order additional tests along with the vitamin B12 test:

  • Complete Blood Count (CBC): Detects anaemia linked to B12 deficiency.
  • Folate Test: Helps distinguish between B12 and folate deficiencies.
  • MMA & Homocysteine: Elevated levels support a B12 deficiency diagnosis.
  • Intrinsic Factor Antibody Test: Checks for pernicious anaemia, a cause of B12 malabsorption.

How to Improve Vitamin B12 Levels

  • Dietary Sources:           

Meat, fish, poultry, eggs, dairy

Fortified cereals and plant-based milks for vegetarians/vegans

  • Supplements:

Oral tablets, capsules, or sublingual (under-the-tongue) forms

Intramuscular injections for severe deficiency or absorption problems

Risks of Untreated Vitamin B12 Deficiency

  • Neurological damage
  • Megaloblastic anaemia
  • Cognitive decline (memory issues, confusion)
  • Fertility issues
  • Osteoporosis and increased fracture risk

Conclusion: Monitoring Your B12 Levels for Long-Term Health

Regular vitamin B12 testing is key to maintaining optimal health, especially if you're at higher risk of deficiency due to age, diet, absorption issues, or medications. Early diagnosis allows prompt treatment, preventing serious complications. If you experience possible deficiency symptoms, ask your doctor about a vitamin B12 test.

Metropolis Healthcare's expert technicians can conveniently collect blood samples at your home for accurate B12 testing at their advanced labs. With reliable results and personalised care, Metropolis empowers you to take charge of your health.

FAQs About Vitamin B12 Test

What is the vitamin B12 test used for?

The vitamin B12 test's purpose is primarily to diagnose vitamin B12 deficiency, monitor treatment effectiveness, and investigate the underlying causes of certain health conditions, such as anaemia and neurological symptoms.

What are the symptoms of vitamin B12 deficiency?

Symptoms of vitamin B12 deficiency can include fatigue, weakness, pale skin, shortness of breath, numbness or tingling in the hands and feet, balance problems, memory loss, and mood changes.

How can I prevent a vitamin B12 deficiency?

You can help prevent vitamin B12 deficiency by consuming a balanced diet that includes B12-rich foods, such as meat, fish, poultry, eggs, and dairy products. If you follow a vegetarian or vegan diet, fortified foods and supplements may be necessary to ensure adequate intake.

Can I take vitamin B12 supplements?

Yes, vitamin B12 supplements are generally safe and well-tolerated. They are available in various forms, including tablets, capsules, liquids, and sublingual preparations. However, it's essential to consult with your healthcare provider before starting any new supplement regimen.

How long does it take to get the results of a vitamin B12 test?

The time it takes to receive your vitamin B12 test results may vary depending on the laboratory and your healthcare provider.

What is the treatment for vitamin B12 deficiency?

Vitamin B12 deficiency is treated with B12 supplements, either orally or through injections, depending on severity.

Can vitamin B12 deficiency be treated?

Yes, it can be effectively treated. If caused by poor diet, oral supplements are enough. In absorption issues like pernicious anaemia, B12 injections or high-dose sublingual tablets may be needed.

Are there any risks associated with the Vitamin B12 Test?

The vitamin B12 test is very safe. Minor pain, bruising, or slight bleeding may occur at the needle site. Rarely, infection may happen.

What happens if I have high levels of vitamin B12?

Though rare, high B12 can signal liver disease, blood disorders, kidney problems, or excess supplementation.

What happens if B12 is low?

Low B12 may cause fatigue, tingling, poor balance, anaemia, or memory issues. Untreated deficiency can lead to permanent nerve damage.

Talk to our health advisor

Book Now

LEAVE A REPLY

Your email address will not be published. Required fields are marked *

Popular Tests

Choose from our frequently booked blood tests

TruHealth Packages

View More

Choose from our wide range of TruHealth Package and Health Checkups

View More
Cart items will be truncated if you change the city.
Do you want to proceed ?