Vitamin D-1,25-Dihydroxy Serum

What is a Vitamin D 1,25-Dihydroxy Test?

The Vitamin D 1,25-Dihydroxy Test measures the level of calcitriol, the biologically active form of vitamin D, in your blood. Calcitriol plays a vital role in maintaining calcium and phosphate balance, supporting bone strength, and regulating parathyroid and kidney function.

Unlike the more common Vitamin D 25-Hydroxy Test, which evaluates overall vitamin D storage, the 1,25-Dihydroxy test helps assess the hormonal activity of vitamin D and how effectively your body converts it into its active form.

Doctors often recommend this test to diagnose or monitor complex metabolic, renal, or endocrine disorders that affect calcium regulation.

What Does the Vitamin D 1,25-Dihydroxy Test Measure?

This test measures the concentration of 1,25-dihydroxyvitamin D (calcitriol) using the Chemiluminescence Immunoassay (CLIA) method.

It helps your doctor to:

• Evaluate calcium and phosphate metabolism
• Diagnose and monitor chronic kidney disease or renal osteodystrophy
• Detect disorders related to parathyroid hormone imbalance (hyperparathyroidism orhypoparathyroidism)
• Assess vitamin D–dependent rickets and metabolic bone diseases
• Evaluate granulomatous disorders such as sarcoidosis and some lymphomas
• Monitor vitamin D therapy and supplementation in renal patients

Who Should Get a Vitamin D 1,25-Dihydroxy Test and Why It’s Done

When is a Vitamin D 1,25-Dihydroxy Test Recommended?

Your doctor may recommend this test if you experience:

• Bone pain or muscle weakness
• Recurrent bone fractures or osteoporosis
• Abnormal calcium or phosphate levels
• Symptoms of kidney disease or hyperparathyroidism
• Rickets or poor bone growth (especially in children)
• Chronic fatigue, confusion, or metabolic imbalance

Who Needs This Test?

The test is particularly useful for:

• Patients with chronic kidney disease (CKD)
• Individuals with parathyroid gland disorders
• People with unexplained calcium imbalance
• Patients undergoing vitamin D therapy
• Those with suspected granulomatous diseases (e.g., sarcoidosis)

Why is a Vitamin D 1,25-Dihydroxy Test Done?

The test helps to:

• Assess active vitamin D function in calcium and phosphate regulation
• Diagnose hyperparathyroidism and renal osteodystrophy
• Monitor treatment for kidney and bone disorders
• Evaluate vitamin D metabolism and absorption problems
• Differentiate causes of calcium abnormalities
• Detect rare conditions such as hereditary vitamin D–resistant rickets

Importance of the Vitamin D 1,25-Dihydroxy Test

The Vitamin D 1,25-Dihydroxy Test is crucial for diagnosing complex calcium metabolism disorders.
It provides detailed insights into how the body converts and uses vitamin D, helping to identify issues with kidney function, parathyroid activity, or bone metabolism.

Monitoring calcitriol levels helps doctors guide treatment for chronic kidney disease, rickets, and certain cancers linked to abnormal calcium levels.

Vitamin D 1,25-Dihydroxy Test Booking and Reports – Metropolis Healthcare

How to Book the Vitamin D 1,25-Dihydroxy Test

1. Online Booking
   Book the test through the Metropolis Healthcare website or mobile app. Choose between home sample collection or visiting your nearest Metropolis Lab.
2. Home Sample Collection
   A trained phlebotomist collects 2–3 ml of serum using a sterile gel vacutainer.
3. Advanced Laboratory Testing
   Testing is conducted using Chemiluminescence Immunoassay (CLIA) in NABL and CAP-accredited Metropolis laboratories for high accuracy.
4. Real-Time Tracking
   Track your sample and view report status on the Metropolis Healthcare app or website.
5. Report Access
   Reports are usually available within 6 hours, delivered via email, WhatsApp, or through your Metropolis Healthcare account.

Is Home Sample Collection Available for the Vitamin D 1,25-Dihydroxy Test?

Yes, Metropolis Healthcare offers home sample collection for the Vitamin D 1,25-Dihydroxy Test.
Certified phlebotomists collect your sample safely at your home under strict hygiene and temperature-controlled conditions.

How Long Does It Take to Get Vitamin D 1,25-Dihydroxy Test Results?

Reports are generally available within 6 hours of sample reaching the lab.
Note: Reporting time and cost may vary depending on your location.

Where Can You Access Your Vitamin D 1,25-Dihydroxy Test Reports?

You can view and download your reports:

• On the Metropolis Healthcare website
• Via the Metropolis Healthcare app
• Through email or WhatsApp
• Or collect a printed copy from your nearest Metropolis Lab

Understanding Vitamin D 1,25-Dihydroxy Test Results

How to Prepare for a Vitamin D 1,25-Dihydroxy Test

To ensure accurate results:

• No fasting is required.
• Inform your doctor about any vitamin D, calcium, or phosphorus supplements.
• Avoid taking biotin (vitamin B7) supplements 8 hours before the test if applicable.
• Share any kidney or hormonal health history with your doctor.

How is the Vitamin D 1,25-Dihydroxy Test Performed?

The procedure is quick and straightforward:

• A blood sample is drawn from a vein in your arm.
• Around 2–3 ml of serum is collected in a gel vacutainer tube.
• The sample is analysed using Chemiluminescence Immunoassay (CLIA) to measure calcitriol levels.
• Results are compared with the biological reference range for accurate diagnosis.

Factors That May Affect Test Accuracy

• Kidney or parathyroid disorders
• Vitamin D or calcium supplementation
• Use of certain medications (e.g., anticonvulsants)
• Improper sample handling or delayed processing
• Hormonal or metabolic imbalances

Diseases Detected by the Vitamin D 1,25-Dihydroxy Test

The test helps diagnose and monitor:

• Chronic kidney disease (CKD)
• Hyperparathyroidism and hypoparathyroidism
• Sarcoidosis and granulomatous diseases
• Osteoporosis and rickets
• Tumour-induced hypercalcaemia
• Hereditary vitamin D–resistant rickets