HLA Typing Test

What Does an HLA Typing Test Measure?

This test analyses the genetic sequences of HLA genes on chromosome 6 that regulate your immune system. It measures:

• HLA Class I genes (A, B, C): These help your immune system recognise infected or foreign cells.
• HLA Class II genes (DR, DQ, DP): These play a key role in immune response regulation and transplant compatibility.
• Genetic compatibility: Determines how closely your HLA profile matches with that of a potential donor.
• Immunological risk: Helps predict the likelihood of transplant rejection or autoimmune conditions.

HLA Typing Test: Who Needs It and Why It’s Done

What Symptoms May Call for an HLA Typing Test?

Although this test isn’t based on symptoms, your doctor may recommend it if you:

• Are being evaluated for a kidney, liver, or heart transplant
• Are a potential bone marrow or stem cell donor or recipient
• Have a family member undergoing transplant evaluation
• Show signs of autoimmune diseases such as celiac disease or ankylosing spondylitis
• Have experienced unexplained organ rejection or severe drug reactions

Who Should Get an HLA Typing Test?

You may need this test if you:

• Are donating or receiving an organ or bone marrow transplant
• Are part of a family genetic screening for transplant compatibility
• Are being assessed for autoimmune conditions related to HLA genes
• Are being evaluated for drug hypersensitivity reactions

Why is an HLA Typing Test Done?

The test helps doctors match donors and recipients with compatible HLA profiles to ensure transplant success. It also helps identify genetic links to autoimmune diseases and predicts adverse drug reactions in susceptible individuals.

Importance of an HLA Typing Test

The HLA typing test is crucial for safe and successful transplantation. It helps reduce the risk of organ rejection, improves graft survival, and allows doctors to personalise immunosuppressive therapy.

High-resolution typing using Next-Generation Sequencing (NGS) provides extremely accurate results, helping to:

• Identify the most compatible donor-recipient pairs
• Support effective transplant planning and management
• Detect genetic patterns linked to autoimmune and hypersensitivity diseases
• Enhance long-term transplant outcomes

HLA Typing Test Booking & Reports – Metropolis Healthcare

How to Book the HLA Typing Test?

1. Simple Online Booking
   Book your test easily through the Metropolis Healthcare website or app. Select the HLA Typing Test, choose your preferred time slot, and provide your details for home collection or lab visit.
2. Safe Home Sample Collection
   Our trained phlebotomists collect your sample using sterile EDTA vacutainers under strict hygiene and safety protocols.
3. Sample Tracking Updates
   You can track your sample status online through the Metropolis Healthcare website or app.
4. Accurate Laboratory Testing
   Your sample is analysed in our NABL & CAP accredited laboratories using advanced Next-Generation Sequencing (NGS) technology for high-resolution HLA identification.
5. Quick & Easy Reports
   Receive your detailed test reports via email, WhatsApp, or download them directly from the Metropolis Healthcare website or mobile app.

Is Home Sample Collection for HLA Typing Test Available Near You?

Yes. Metropolis Healthcare offers doorstep sample collection for HLA typing. Our team ensures accurate collection and safe transport of your sample, saving you time and reducing hospital visits.

In how much time will I get HLA Typing Test report?

Reports are usually available within 7 days after the sample reaches the laboratory.
Note: Reporting time and cost may vary depending on your location.

Where can I see or get HLA Typing Test results?

You can access your reports securely through the Metropolis Healthcare website or app. Results are also shared via email and WhatsApp, and printed copies can be collected from your nearest Metropolis lab.

Interpreting HLA Typing Test Results

What Your HLA Typing Test Results May Indicate

Understanding Abnormal HLA Typing Test Results by Parameter

How Should You Prepare for an HLA Typing Test?

To ensure a smooth test experience, keep these points in mind:

• No fasting required: You can eat and drink normally before the test.
• Medication disclosure: Inform your doctor about any ongoing treatments, transfusions, or immune-modifying drugs.
• Clinical details: Provide your medical and family history, along with your clinician’s contact number.
• No transfusions: Avoid non-leucodepleted blood transfusions for at least 14 days before the test to prevent result interference.
• Stay hydrated: Maintain normal fluid intake before sample collection.

How Is an HLA Typing Test Done? (Step-by-Step Procedure)

• Preparation: A tourniquet is applied to make the vein visible.
• Cleaning: The puncture area is cleaned with an antiseptic.
• Blood Draw: 6 ml of blood is drawn into an EDTA vacutainer.
• Lab Processing: DNA is extracted from your blood and HLA genes are amplified using advanced PCR methods.
• Sequencing: Next-Generation Sequencing (NGS) is used to identify your HLA alleles with high precision.
• Analysis: Results are compared against the IMGT/HLA database to determine your genetic profile.

Conditions That May Affect HLA Typing Test Accuracy

Several factors may influence test accuracy:

• Recent blood transfusions: Can alter DNA profile and cause mismatched results.
• Incomplete medical details: Missing clinical history can delay interpretation.
• Technical limitations: Rare allele variants may occasionally cause ambiguity in typing results.

Diseases That an HLA Typing Test Can Help Detect

The HLA typing test aids in identifying and managing:

• Organ and bone marrow transplant compatibility
• Autoimmune diseases such as celiac disease and type 1 diabetes
• Rheumatological conditions like ankylosing spondylitis
• Drug hypersensitivity syndromes (e.g., abacavir sensitivity)
• Immune response disorders and genetic susceptibility screening

HLA Typing Test Price in Different Cities

The cost of a HLA Typing Test may vary depending on your location. Here's a table showing the approximate price range for the HLA Typing Test at Metropolis Healthcare in major Indian cities:

The HLA Typing Test cost may vary depending on the city and the specific tests included in the panel. However, Metropolis Healthcare offers competitive HLA Typing Test prices across major cities in India.

References

1. Blais J., et al. (2015). Risk of Misdiagnosis Due to Allele Dropout and False-Positive PCR Artifacts in Molecular Diagnostics. Journal of Molecular Diagnostics, 17(5), 505–514. PMID: 26115770
2. Steiner N.K., Hou L., Hurley C.K. (2018). Characterizing alleles with large deletions using region specific extraction. Human Immunology, 79(6), 491–493. PMID: 29698748
3. Petersdorf E.W., et al. (2014). The role of HLA matching in hematopoietic cell transplantation. New England Journal of Medicine, 371(4), 339–348. PMID: 25054722
4. Robinson J., et al. (2020). The IPD-IMGT/HLA Database: HLA typing and allele frequency resources. Nucleic Acids Research, 48(D1), D948–D955. PMID: 31624801