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AFB XDR Screen- Sputum / BAL/ Other respiratory samples

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AFB Test Overview

Tuberculosis is a chronic respiratory tract infection causing fever, cough and weight loss. It is commonly caused by Mycobacterium tuberculosis (M.tb). Many diagnostic techniques are available for diagnosis of tuberculosis which include staining, culture and nucleic acid detection. Among nucleic acid detection techniques are polymerase chain reaction (PCR), Cartridge based nucleic acid amplification technique (CBNAAT), Line probe assay (LPA), Next generation sequencing (NGS).


With increase in drug resistance in tuberculosis patient many patients would require testing of second line drugs.
LPA is based on DNA strip technology. It is approved by WHO for testing pulmonary samples and M.tb culture isolates. 
In addition to detection of M.tb it can detect resistance to quinolone and aminoglycoside group of antibiotics

Written by: Dr Vishal Wadhwa, M.D, D.N.B Microbiology, Medical Affairs

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AFB XDR Screen- Sputum / BAL/ Other respiratory samples Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the AFB XDR Screen- Sputum / BAL/ Other respiratory samples with a clear pricing structure.

The AFB XDR Screen- Sputum / BAL/ Other respiratory samples Price in Hyderabad is ₹ 5,705 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

This test has been approved by WHO and also by Govt. of India as a follow up test post detection of resistance by first line LPA and Xpert Mtb/Rif. 
In addition to detection of M.tb it can detect resistance to quinolone (levofloxacin, moxifloxacin) and aminoglycoside (amikacin, Kanamycin, capreomycin, viomycin) group of antibiotics

This test detects DNA of Mtb and mutations which can lead to resistance towards levofloxacin, moxifloxacin, amikacin, Kanamycin, capreomycin and viomycin.

During the test patient will be advised to rinse the mouth with clean water and then expectorate sputum with a deep cough. Aim is to get secretions from the lower respiratory tract which are not contaminated with sputum. Sputum sample will be collected in a screw capped container.
Test can be booked if pure culture of M.tb is provided at the time of booking

M.tb detected - This means that the DNA of M.tb is present in sample and patient is likely to be suffering from tuberculosis.
M.tb Not detected - This means that the DNA of M.tb is absent in sample and patient is unlikely to suffer from tuberculosis. However, sensitivity of this test is not 100%. Repeat test on fresh sample or culture may be advised by doctor to the patient
Resistance to Fluoroquinolone detected - This implies that patient is unlikely to respond to fluoroquniolone treatment. However confirmation with phenotypic is advisable
Resistance to Aminoglycosides detected- This implies that patient is unlike to respond to aminoglycoside treatment (check report for comments on individual antibiotics from aminoglycosides class). However confirmation with phenotypic is advisable

There is no need to get ready for the test other than to rinse your mouth thoroughly with water before taking a sputum sample.

The patient will be instructed to rinse their mouth with water before coughing vigorously to expel mucus during the test. The goal is to collect lower respiratory tract secretions free of sputum contamination. A sputum sample will be collected In a jar with a screw-on lid.

If a pure culture of M.TB is submitted at the time of booking, a test can be scheduled.

 

Based on the initial findings, the following tests might be recommended -

  1. ZN Stain.
  2. Sputum Culture.
  3. Pleural Fluid for AFB.
  4. Bronchial Washing.
  5. MTB PCR      
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