AFB-DNA (TB-PCR) Detection – Sputum/BAL
Overview
AFB-DNA (TB-PCR) detection using Real-Time PCR is a molecular testing method aimed at identifying the presence of Mycobacterium tuberculosis (MTB) complex DNA in sputum or bronchoalveolar lavage (BAL) samples. This test enables the accurate diagnosis of MTB infection, aiding in the early detection and management of tuberculosis (TB). Additionally, if the initial result is MTB-positive, further testing is conducted using the Ultra Cartridge-Based Nucleic Acid Amplification Test (CBNAAT) to determine rifampicin resistance. This approach allows for the rapid identification of drug-resistant TB strains. Moreover, the test can distinguish between MTB complex and non-tuberculosis bacteria (NTM or MOTT), contributing to the differentiation of TB from other respiratory infections. By providing precise diagnostic information, this testing strategy facilitates timely and appropriate medical interventions, enhancing patient care and public health efforts.
AFB-DNA (TB-PCR) Detection – Sputum/BAL Price
Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the AFB-DNA (TB-PCR) Detection – Sputum/BAL with a clear pricing structure.
The AFB-DNA (TB-PCR) Detection – Sputum/BAL Price in Kodungallur is ₹ 2,250 .
We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.
Frequently Asked Questions
This test measures the presence of genetic material from Mycobacterium tuberculosis (MTB) complex or non-tuberculous bacteria (NTM or MOTT) in respiratory samples.
- To diagnose active tuberculosis (TB) infections caused by MTB complex or other bacterial species.
- To differentiate between TB and non-TB respiratory infections.
- To guide appropriate treatment decisions.
- To determine if the TB bacteria are resistant to rifampicin, a common anti-TB medication.
This test is recommended for individuals who :
- Have symptoms of respiratory infections, such as chronic cough, coughing up blood, chest pain, or shortness of breath.
- Are suspected to have TB based on clinical evaluation or exposure to TB patients.
- Need confirmation of TB diagnosis for appropriate treatment initiation.
- Require detection of rifampicin resistance in MTB for effective management.
- An abnormal, i.e., positive result indicates the presence of MTB complex or other bacteria, confirming active TB or non-TB respiratory infection.
- If positive for MTB complex, reflex testing for rifampicin resistance will be performed. A negative result suggests the absence of MTB complex or other bacteria, but does not rule out TB entirely.
During this test, you will provide a sputum or bronchoalveolar lavage (BAL) sample in a sterile container which will be sent for analysis.
Please reach out to your healthcare provider for any specific requirements.
- Acid-fast bacilli (AFB) smear microscopy: It examines sputum samples for the presence of acid-fast bacteria, including MTB.
- Chest X-ray: It helps visualise lung abnormalities suggestive of TB or other respiratory conditions.
- Drug susceptibility testing: It determines the susceptibility of MTB to various anti-TB medications.
TB-PCR test, Mycobacterial DNA detection by real-time PCR
Tuberculosis (TB) is a contagious infection caused by Mycobacterium tuberculosis (MTB) complex bacteria. It primarily affects the lungs but can also involve other organs. TB can be transmitted through the air when an infected person coughs, sneezes, or speaks.
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Why Metropolis?
Metropolis has a team of 200 senior pathologists and over 2000 technicians delivering diagnostic solutions in the areas of routine, semi specialty and super specialty domains like Oncology, Neurology, Gynaecology, Nephrology and many more.
We offer a comprehensive range of 4000+ clinical laboratory tests and profiles, which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease.

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