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Vitamin B6 Test

Also known as: Vitamin B6 Pyridoxine Test

51+ booked in last 3 days

Vitamin B6 Pyridoxine Test Details in Brief

Also Known As Pyridoxine Test, Vitamin B6 (Pyridoxal Phosphate) Test
Purpose Helps diagnose vitamin B6 deficiency or toxicity, evaluate neurological symptoms, assess nutritional status, and monitor therapy for certain metabolic or anaemic conditions
Preparation Avoid taking vitamin B6 supplements for at least 24 hours before the test
Fasting Fasting not required
Reporting Time 1 Week
Cost 3,700

What is a Vitamin B6 Test?

The Vitamin B6 Test measures the amount of pyridoxal phosphate (PLP) — the active form of vitamin B6 — in your blood. Vitamin B6 plays a key role in metabolism, brain function, mood regulation, and immune health.

A deficiency can lead to symptoms such as fatigue, anaemia, skin inflammation, or nerve problems, while excess levels may cause nerve toxicity.

This test is done using EDTA blood and analysed through High-Performance Liquid Chromatography (HPLC), a highly accurate method used to measure vitamin levels.

What Does a Vitamin B6 Test Measure?

The test helps assess how well your body is using vitamin B6 by measuring the concentration of pyridoxal phosphate in your blood. It provides important insights into:

  • Vitamin B6 Deficiency or Toxicity: Detects low or high levels affecting nerve and metabolic functions.
  • Neurological Health: Helps evaluate causes of tingling, numbness, or nerve damage.
  • Nutritional Assessment: Assesses overall nutritional status and vitamin absorption.
  • Drug Interaction Monitoring: Detects drug-induced vitamin B6 depletion caused by certain medications.
  • Anaemia Evaluation: Supports diagnosis of sideroblastic anaemia and other B6-responsive anaemias.

Vitamin B6 Test: Who Needs It and Why It’s Done

What Symptoms May Call for a Vitamin B6 Test?

Your doctor may recommend a Vitamin B6 test if you experience:

  • Tingling, numbness, or burning sensations in hands or feet
  • Fatigue or unexplained weakness
  • Mood changes, irritability, or depression
  • Cracked lips, sore tongue, or skin rashes
  • Poor immunity or frequent infections
  • Symptoms of anaemia, such as paleness or shortness of breath

Who Should Get a Vitamin B6 Test?

The test is especially useful for:

  • Individuals showing signs of vitamin deficiency or toxicity
  • Patients on medications such as isoniazid, cyclosporine, or penicillamine that affect vitamin B6 levels
  • People with neurological symptoms or metabolic disorders
  • Pregnant or lactating women being monitored for nutritional balance
  • Patients with anaemia not responding to iron therapy

Why is a Vitamin B6 Test Done?

The Vitamin B6 Test helps doctors identify whether your symptoms are related to vitamin deficiency, drug interference, or metabolic issues. It also assists in personalising nutritional or supplementation therapy for long-term health management.

Importance of a Vitamin B6 Test

Vitamin B6 is essential for the production of neurotransmitters like serotonin and for red blood cell formation. Measuring its levels helps detect underlying causes of anaemia, nerve damage, or mood disorders early.

By evaluating both deficiency and excess, the test guides effective treatment, prevents neurological complications, and ensures optimal nutrient balance, particularly in patients with metabolic or autoimmune conditions.

Vitamin B6 Test Booking & Reports – Metropolis Healthcare

How to Book the Vitamin B6 Test and Get Your Reports?

  1. Simple Online Booking
    You can book the Vitamin B6 Test through the Metropolis Healthcare website or app. Select the test, choose your preferred time slot, and share your address for home sample collection. You can also visit the nearest Metropolis Lab to provide your sample directly.
  2. Safe Home Sample Collection
    Our trained phlebotomists collect your blood sample safely and ensure proper light protection of the tube using aluminium foil, as required for accurate results.
  3. Sample Tracking Updates
    You can track your sample’s progress from collection to processing via the Metropolis Healthcare website or app.
  4. Accurate Laboratory Testing
    Samples are processed in NABL & CAP accredited laboratories using High-Performance Liquid Chromatography (HPLC) for precise and reliable measurement.
  5. Quick & Easy Reports
    Reports are delivered promptly through email, WhatsApp, or can be downloaded directly from the Metropolis Healthcare app or website.

Is Home Sample Collection for Vitamin B6 Test Available Near You?

Yes. Metropolis Healthcare offers home sample collection for the Vitamin B6 Test at your convenience. A certified phlebotomist will visit your home, collect the sample under safe conditions, and ensure it is properly shielded from light to maintain accuracy.

How Long Does It Take to Get a Vitamin B6 Test Report?

Reports are usually available after 1 week once your sample reaches the lab.
Note: Reporting time may vary based on your location.

Where Can I See or Get Vitamin B6 Test Results?

Your reports are easily accessible via:

  1. The Metropolis Healthcare website (login with your credentials)
  2. The Metropolis Healthcare App
  3. Email or WhatsApp delivery
  4. Physical copies available at your nearest Metropolis Lab

Interpreting Vitamin B6 Test Results

What Your Vitamin B6 Test Results May Indicate

Parameter

Normal Range

High Values May Indicate

Low Values May Indicate

Vitamin B6 (Pyridoxal Phosphate)

8.7 – 27.2 ng/mL

Vitamin B6 toxicity, excessive supplementation

Vitamin B6 deficiency, poor absorption, medication effect, alcoholism, or chronic illness

 

Understanding Abnormal Vitamin B6 Results by Parameter

Parameter

High Values May Indicate

Low Values May Indicate

Vitamin B6 (Pyridoxal Phosphate)

Excessive vitamin intake, supplement overuse, or toxicity leading to nerve issues

Nutritional deficiency, drug-induced depletion, anaemia, or metabolic dysfunction

 

How Should You Prepare for a Vitamin B6 Test?

To ensure accurate results, follow these simple steps before your test:

  • Avoid supplements: Do not take vitamin B6 supplements for at least 24 hours before your test.
  • No fasting required: You may eat and drink normally.
  • Medication disclosure: Inform your doctor about any medications you are taking, especially those that affect vitamin metabolism.
  • Sample protection: The sample must be protected from light, so the collection tube will be wrapped in aluminium foil by the technician.
  • Stay hydrated: Drink sufficient water before your test to help with easy blood collection.

How Is a Vitamin B6 Test Done? (Step-by-Step Procedure)

The Vitamin B6 Test is conducted through a simple blood draw process:

  • Preparation: A tourniquet is tied around your upper arm to make veins more visible.
  • Cleaning: The puncture site is cleaned using antiseptic.
  • Sample Collection: A sterile needle is used to collect about 2–3 ml of blood into an EDTA vacutainer, which is then covered with aluminium foil to protect it from light.
  • Completion: The needle is removed, and a bandage is applied to your arm.
  • Processing: The sample is tested using High-Performance Liquid Chromatography (HPLC) to determine vitamin B6 levels.

Conditions That May Affect Vitamin B6 Test Accuracy

Certain factors can temporarily alter vitamin B6 levels:

  • Recent vitamin supplementation: Taking vitamin B6 tablets or multivitamins before the test can give falsely high results.
  • Medication use: Drugs like isoniazid, cyclosporine, or penicillamine can lower vitamin B6 levels.
  • Alcohol consumption: Chronic alcohol use may reduce vitamin absorption.
  • Pregnancy or lactation: Can slightly decrease blood vitamin B6 levels due to increased nutritional demand.
  • Severe illness or stress: May influence metabolism and affect test results.

Diseases That a Vitamin B6 Test Can Help Detect

The Vitamin B6 Test helps identify or manage:

  • Vitamin B6 deficiency or toxicity
  • Peripheral neuropathy and nerve disorders
  • Sideroblastic anaemia
  • Depression or mood-related disorders
  • Metabolic disorders
  • Drug-induced nutrient depletion
  • Chronic alcoholism or malnutrition

Vitamin B6 Test Price in Different Cities

The cost of a Vitamin B6 Test may vary depending on your location. Here's a table showing the approximate price range for the Vitamin B6 Test at Metropolis Healthcare in major Indian cities:

City

Approximate Cost (INR)

Mumbai

₹ 3,500 to ₹ 3,700

Delhi

₹ 3,500 to ₹ 3,700

Bengaluru

₹ 3,500 to ₹ 3,700

Chennai

₹ 3,500 to ₹ 3,700

The Vitamin B6 Test cost may vary depending on the city and the specific tests included in the panel. However, Metropolis Healthcare offers competitive Vitamin B6 Test prices across major cities in India.

References

  1. Bender, D. A. (1994). Novel functions of vitamin B6. Proceedings of the Nutrition Society, 53(3), 625–630. PMID: 7886055
  2. Dakshinamurti, K., & Dakshinamurti, S. (2007). Vitamin B6: Metabolism, functions, and clinical implications. Molecular Aspects of Medicine, 28(1), 1–13. PMID: 17207913
  3. Leklem, J. E. (1990). Vitamin B6: A status report. Journal of Nutrition, 120(Suppl 11), 1503–1507. PMID: 2243301
  4. Di Salvo, M. L., Contestabile, R., & Safo, M. K. (2011). Vitamin B6 salvage enzymes: Mechanism, structure, and regulation. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics, 1814(11), 1597–1608. PMID: 21784152
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Vitamin B6 Pyridoxine Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Vitamin B6 Pyridoxine Test with a clear pricing structure.

The Vitamin B6 Pyridoxine Test Price in Mumbai is ₹ 3,700 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

It measures the level of pyridoxal phosphate (PLP), the active form of vitamin B6, to assess deficiency or excess affecting nerves and metabolism.

Yes, avoid taking vitamin B6 supplements for at least 24 hours before the test to ensure accuracy.

No, fasting is not required. You can eat and drink normally before your test.

A small blood sample is drawn from your vein into a light-protected EDTA tube and tested using HPLC technology.

Fatigue, tingling in hands or feet, cracked lips, mood changes, and poor immunity may indicate deficiency.

Yes, drugs such as isoniazid, cyclosporine, and penicillamine can lower your vitamin B6 levels.

Normal values typically range between 8.7 – 27.2 ng/mL.

High levels usually indicate excessive supplement intake and can lead to nerve-related toxicity.

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We offer a comprehensive range of 4000+ clinical laboratory tests and profiles, which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease.

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