Her-2 Neu gene amplification, FFPE Tissue

The detection of Her-2/Neu (C-Er-B2) status by fluorescence in situ hybridization (FISH) is a critical aspect in the diagnosis and treatment planning of certain cancers, most notably breast cancer. Her-2/neu is a proto-oncogene that encodes a receptor tyrosine kinase protein, and its overexpression or amplification is linked to aggressive tumor behavior and resistance to conventional therapies. Fluorescence in situ hybridization, or FISH, is a molecular diagnostic technique that allows for the visualization and quantification of specific DNA sequences, in this case, the ERBB2 gene, on a cellular level. By assessing Her-2/Neu status through FISH, clinicians gain valuable information about the cancer's molecular profile, aiding in the selection of targeted therapies such as Herceptin. This personalized approach enhances treatment efficacy and improves patient outcomes, exemplifying the pivotal role of Her-2/Neu (C-Er-B2) status determination by FISH in the management of certain malignancies.