Factor V Leiden Mutation Test

What Does a Factor V Leiden Mutation Test Measure?

The test analyzes the patient’s DNA to detect the G1691A point mutation in the Factor V (F5) gene.

• Normal (Negative): No mutation present; normal clotting function.
• Heterozygous Mutation: One copy of the mutant gene; moderate risk of abnormal clot formation.
• Homozygous Mutation: Two copies of the mutant gene; high risk of recurrent or severe thrombotic events.

Factor V Leiden Mutation Test: Who Needs It and Why It’s Done

Who Should Get a Factor V Leiden Mutation Test?

Doctors may recommend this test for individuals who:

• Have had unexplained blood clots (DVT or PE), especially before age 50
• Have a family history of thrombosis or Factor V Leiden mutation
• Have recurrent miscarriages or pregnancy complications linked to clotting
• Have thrombosis in unusual locations (e.g., cerebral, mesenteric, or portal veins)
• Are undergoing hormone therapy or oral contraceptive use with a personal/family history of clotting
• Are planning major surgery with increased clotting risk

Why is a Factor V Leiden Mutation Test Done?

This test helps:

• Confirm hereditary thrombophilia (genetic clotting disorder)
• Determine the cause of unexplained blood clots
• Guide anticoagulation therapy or preventive treatment
• Evaluate pregnancy-related complications due to clotting
• Screen family members for inherited risk

Importance of a Factor V Leiden Mutation Test

The Factor V Leiden mutation is the most common genetic cause of inherited thrombophilia and is found in up to 5% of the general population. Identifying this mutation helps clinicians:

• Predict future clotting risk
• Tailor preventive treatments, including anticoagulants
• Provide lifestyle and medication guidance (e.g., avoiding estrogen-based contraceptives)
• Reduce risk of recurrent thrombosis and related complications

Factor V Leiden Mutation Test Booking & Reports – Metropolis Healthcare

How to Book the Factor V Leiden Mutation Test and Get Your Reports

1. Simple Online Booking
   Book the test on the Metropolis Healthcare website or app. Choose the Factor V Leiden Mutation Test, select a time slot, and opt for home sample collection or visit your nearest Metropolis Lab.
2. Safe Sample Collection
   A phlebotomist collects a 3 ml EDTA blood sample in a sterile EDTA vacutainer, following hygiene and safety protocols.
3. Sample Tracking Updates
   Track your sample’s progress in real time via the Metropolis Healthcare app or website.
4. Accurate Laboratory Testing
   Samples are analyzed in NABL & CAP accredited laboratories using Real-Time PCR (RT-PCR) for precise mutation detection.
5. Quick & Easy Reports
   Get your reports the same day through email, WhatsApp, or the Metropolis Healthcare website.

Is Home Sample Collection for Factor V Leiden Mutation Test Available Near You?

Yes. Metropolis Healthcare offers doorstep blood collection for this test. A trained technician will visit your location for safe and convenient sample collection.

How Long Does It Take to Get a Factor V Leiden Mutation Test Report?

Reports are usually available on the same day after sample reaces the lab.
Note: Reporting time may vary based on location.

Where Can You View or Collect Your Factor V Leiden Mutation Test Results?

Reports can be accessed through the Metropolis Healthcare website, mobile app, email, or WhatsApp. Physical copies are also available at your nearest Metropolis lab.

Interpreting Factor V Leiden Mutation Test Results

What Your Factor V Leiden Mutation Test Results May Indicate

Understanding Abnormal Factor V Leiden Mutation Test Results by Parameter

How Should You Prepare for a Factor V Leiden Mutation Test?

• No fasting required – you can eat and drink normally.
• Provide medical history – mention any personal or family history of clotting.
• Inform your doctor about any ongoing anticoagulant or hormonal therapy.
• Stay hydrated before sample collection to ensure a smoother blood draw.

How Is a Factor V Leiden Mutation Test Done? (Step-by-Step Procedure)

1. Sample Collection: 3 ml of blood is drawn into an EDTA vacutainer.
2. DNA Extraction: DNA is isolated from the blood sample.
3. Mutation Detection: The F5 gene is analyzed for the G1691A mutation using RT-PCR.
4. Result Interpretation: The lab determines if the mutation is absent, heterozygous, or homozygous.
5. Report Delivery: Results are generated and shared electronically.

Conditions That May Affect Factor V Leiden Mutation Test Accuracy

• Contaminated or hemolyzed blood sample
• Inadequate sample volume
• Improper sample storage temperature
• Technical errors or PCR inhibition

Diseases That a Factor V Leiden Mutation Test Can Help Detect

• Hereditary thrombophilia
• Deep vein thrombosis (DVT)
• Pulmonary embolism (PE)
• Recurrent miscarriages due to clotting disorders
• Cerebral or portal vein thrombosis

Factor V Leiden Mutation Test​ Price in Different Cities

The cost of a Factor V Leiden Mutation Test​ may vary depending on your location. Here's a table showing the approximate price range for the Factor V Leiden Mutation Test​ at Metropolis Healthcare in major Indian cities:

The Factor V Leiden Mutation Test​ cost may vary depending on the city and the specific tests included in the panel. However, Metropolis Healthcare offers competitive Factor V Leiden Mutation Test​ prices across major cities in India.

References

1. Reitter-Pfoertner S. (2012). Factor V Leiden Mutation and Thrombophilia. Blood Reviews, 26(1), 37–45. PMID: 22018618
2. Gaunt T.R., et al. (2012). The impact of Factor V Leiden mutation on venous thromboembolism risk. European Journal of Human Genetics, 21(3), 283–289. PMID: 23222682
3. Ridker P.M., et al. (1995). Mutation in the gene coding for coagulation Factor V and the risk of thrombosis. New England Journal of Medicine, 332(14), 912–917. PMID: 7860930