Sperm DNA Fragmentation (DFI)

Sperm DNA Fragmentation measures the extent of DNA damage in sperm. It helps evaluate the genetic integrity of sperm and determine if there is excessive fragmentation that could affect fertility. This test provides valuable information about the potential for successful conception and can help guide appropriate treatment options.

This test is done:

• To assess male fertility and identify potential causes of infertility
• To understand the underlying causes of unexplained infertility or recurrent miscarriages
• To evaluate the impact of clinical varicocele on sperm DNA integrity
• To determine if poor semen parameters or multiple failed IVF/ICSI treatments are due to DNA fragmentation in sperm

This test is recommended for individuals who experience any of the following:

• Unexplained infertility, where standard semen analysis results are normal
• Recurrent miscarriages without any apparent cause
• Clinical varicocele, a condition where veins in the scrotum are enlarged
• Poor semen parameters such as low sperm count, motility, or morphology
• Multiple failed IVF/ICSI treatments

The normal range for sperm DNA fragmentation is typically less than 15%. If the results show higher levels of fragmentation, it may indicate decreased fertility potential. However, abnormal results do not automatically mean infertility or other serious conditions. It is important to discuss the results with your doctor, who will consider your medical history and other factors to provide a comprehensive interpretation.

High levels of fragmentation may suggest a need for further evaluation or specific treatments.

During the test:

• You will need to provide a freshly collected semen sample in a sterile container.
• The sample should reach the lab within 4 hours at ambient temperature or in frozen condition if more than 4 hours.
• The laboratory will use the Sperm Chromatin Dispersion Method (SCD) to assess DNA fragmentation levels.

Before the test:

• A routine semen analysis report is necessary as a prerequisite.
• You will need a doctor's prescription and should provide any relevant medical history.
• Maintain 3 days of abstinence before collecting the semen sample.
• Reach out to your healthcare provider for any specific requirements.

Other tests that may be ordered alongside the Sperm DNA Fragmentation (DFI) test include:

• Hormone tests to evaluate hormone levels related to fertility
• Genetic testing to identify any underlying genetic causes of infertility
• Ultrasound imaging to assess the structure of the reproductive organs

SCD test (Sperm Chromatin Dispersion) or DNA fragmentation test

Unexplained infertility refers to the inability to conceive without any identifiable cause. It occurs when diagnostic tests do not reveal any specific reasons for infertility in both partners. the Sperm DNA Fragmentation (DFI) test test can provide insights into unexplained infertility by evaluating the genetic integrity of sperm.