HLA-B*27 PCR

What is an HLA B27 PCR Test?

The HLA B27 PCR Test is a genetic blood test that detects the presence of the Human Leukocyte Antigen B27 (HLA-B27) gene in your DNA. This gene is associated with an increased risk of developing certain autoimmune and inflammatory conditions, such as Ankylosing Spondylitis, Reactive Arthritis, and Juvenile Idiopathic Arthritis.

The test uses Polymerase Chain Reaction (PCR) technology to amplify and detect specific DNA sequences accurately, making it highly sensitive and reliable. Although carrying the HLA-B27 gene increases your risk, not everyone with the gene develops disease.

What Does an HLA B27 PCR Test Measure?

The test measures the presence or absence of the HLA-B27 gene in your white blood cells using PCR analysis.

It helps your doctor to:

• Detect genetic susceptibility to autoimmune diseases.
• Support diagnosis of conditions like Ankylosing Spondylitis or Reactive Arthritis.
• Differentiate between inflammatory and non-inflammatory causes of joint pain.
• Assess family members with similar symptoms or a history of autoimmune disorders.

HLA B27 PCR Test: Who Needs It and Why It’s Done

What Symptoms May Call for an HLA B27 PCR Test?

Your doctor may recommend this test if you experience symptoms suggestive of inflammatory joint disease such as:

• Persistent lower back pain or stiffness, especially in the morning.
• Swelling or pain in large joints like hips, knees, or shoulders.
• Inflammation of the eyes (uveitis) causing redness or blurred vision.
• Fatigue or general discomfort associated with autoimmune activity.

Who Should Get an HLA B27 PCR Test?

You may be advised to take this test if you:

• Have a family history of autoimmune conditions.
• Show early signs of spondyloarthropathies such as Ankylosing Spondylitis.
• Are being evaluated for recurrent joint inflammation or chronic back pain.
• Are diagnosed with uveitis or reactive arthritis for confirmation.
• Are under investigation for autoimmune-related inflammatory disorders.

Why is an HLA B27 PCR Test Done?

The HLA B27 PCR Test helps determine whether you carry the HLA-B27 gene that can trigger abnormal immune responses. It assists doctors in diagnosing and monitoring autoimmune conditions and in differentiating genetic predisposition from acquired diseases.

Importance of an HLA B27 PCR Test

Identifying the HLA-B27 gene early can help guide your doctor in making an accurate diagnosis and initiating appropriate management strategies. While the gene itself does not cause disease, its presence indicates an increased risk for developing autoimmune disorders. Knowing your HLA-B27 status helps in monitoring symptoms, predicting disease patterns, and choosing targeted treatments to reduce inflammation and improve long-term joint health.

HLA B27 PCR Test Booking & Reports – Metropolis Healthcare

How to Book the HLA B27 PCR Test and Get Your Reports?

1. Simple Online Booking
   Book the HLA B27 PCR Test through the Metropolis Healthcare website or mobile app. Select your preferred time slot and choose home collection or lab visit as per convenience.
2. Safe Home Sample Collection
   Our trained phlebotomists collect your sample following all hygiene and safety protocols, ensuring a smooth and comfortable experience.
3. Sample Tracking Updates
   You can track your sample and report status through the Metropolis Healthcare portal at every stage.
4. Accurate Laboratory Testing
   Your sample is analysed using advanced PCR technology in NABL and CAP accredited laboratories for precise genetic detection.
5. Quick & Easy Reports
   Reports are typically available within 24 hours via email, WhatsApp, or through the Metropolis Healthcare app and website.

Is Home Sample Collection for HLA B27 PCR Test Available Near You?

Metropolis Healthcare offers convenient home sample collection for the HLA B27 PCR Test. Our phlebotomists collect samples from your doorstep using sterile techniques, ensuring accuracy and patient comfort. This is especially helpful for patients with limited mobility or ongoing inflammation.

How Long Does It Take to Get an HLA B27 PCR Test Report?

Reports are usually available within 24 hours once the sample reaches the laboratory.
Note: Reporting time and cost may vary depending on your location.

Where Can I See or Get HLA B27 PCR Test Results?

You can easily access your test results through:

• The Metropolis Healthcare website or app.
• Email or WhatsApp once your report is ready.
• Collecting a physical copy from your nearest Metropolis Lab.

Interpreting HLA B27 PCR Test Results

What Your HLA B27 PCR Test Results May Indicate

Understanding Abnormal HLA B27 PCR Test Results by Parameter

How Should You Prepare for an HLA B27 PCR Test?

The HLA B27 PCR Test requires no special preparation. Follow these steps to ensure a smooth process:

• No fasting required – you can eat and drink normally.
• Inform your doctor about all current medications and supplements.
• Stay relaxed and hydrated before your appointment.
• Wear a short-sleeved shirt for easier blood draw access.

How Is an HLA B27 PCR Test Done? (Step-by-Step Procedure)

The HLA B27 PCR Test follows a simple, standard blood collection process:

• Preparation: An elastic band is tied around your upper arm to make the vein visible.
• Cleaning: The puncture site is cleaned with antiseptic to prevent infection.
• Blood Draw: A sterile needle is inserted into your vein to collect blood.
• Collection: About 3 ml of blood is drawn into an EDTA vacutainer.
• Completion: The needle is removed, and a bandage is applied.
• Processing: The sample is analysed using Real Time PCR (RT-PCR) to detect the HLA-B27 gene.

Conditions That May Affect HLA B27 PCR Test Accuracy

Although the HLA B27 PCR Test is highly accurate, a few factors may influence results:

• Sample contamination during collection or handling.
• Technical variations in PCR amplification.
• Recent blood transfusion or bone marrow transplant.
• Low DNA concentration or quality in the sample.

Diseases That an HLA B27 PCR Test Can Help Detect

The HLA B27 PCR Test helps doctors identify genetic susceptibility to several autoimmune and inflammatory diseases, including:

• Ankylosing Spondylitis
• Reactive Arthritis
• Psoriatic Arthritis
• Juvenile Idiopathic Arthritis
• Uveitis (inflammatory eye disease)