BCR-ABL Breakpoint Detection by RT-PCR, EDTA Blood

BCR-ABL Breakpoint Analysis Qualitative by Real-Time PCR, performed on blood samples, is a diagnostic test used to diagnose cases of Chronic Myeloid Leukemia (CML). The test has clinical utility in detecting major, minor, and micro translocations of the BCR-ABL fusion gene, which is characteristic of CML. CML is a myeloproliferative neoplasm caused by the fusion of the BCR and ABL genes, resulting in the production of the BCR-ABL fusion protein. By analyzing the breakpoints of the BCR-ABL fusion gene using real-time PCR, this test confirms the presence of the fusion gene and aids in the diagnosis of CML. The detection of major, minor, and micro translocations provides valuable information regarding the specific genetic alterations involved in the disease. This information is essential for determining appropriate treatment strategies and monitoring disease progression in CML patients. Performing the analysis on blood samples offers a less invasive and more convenient option for diagnosing CML compared to bone marrow testing.