HLA High Resolution Typing (A, B, C, DR, DQ, DP) - BMT/ Stem cell Transplant Gene Panel by NGS, EDTA Blood

HLA Typing Test Overview

The HLA (Human Leukocyte Antigen) typing test is a specialised genetic test that identifies the specific genes responsible for encoding the major histocompatibility complex (MHC) proteins. These proteins play a crucial role in regulating the immune system's response to foreign substances, making them essential for determining the compatibility of organ and tissue transplants.

NextGen-HLA typing is a Next Generation Sequencing (NGS) based high-resolution identification of Human Leucocyte Antigen (HLA). The HLA system is a complex of genes on chromosome 6, which encode cell-surface proteins responsible for regulation of the immune system also known as Major histocompatibility complex (MHC) proteins. There are two distinct classes. Class I (A, B, C) & Class II (DR, DQ & DP). HLA genes are highly polymorphic and play an important role in immune – mediated diseases, tumour development processes, transplanted organ or tissue survival determination and drug hypersensitivity. They are particularly important in the case of transplanted tissue, because it can lead to transplant rejection & hence the HLA typing is done to determine HLA match between the Donor & the Recipient. NGS offers Higher & better match between donor & recipient influencing successful outcomes for Hematopoietic stem cell (HSCT) & any solid organ transplant. NGS helps in removing all ambiguity & provides most accurate characterisation of alleles. All the 6 loci shall be reported with resolution up to 6 digits.

Written by - Dr. Shibani R, Medical Writer, Medical Affairs