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FSH LH Prolactin Testosterone Profile Test

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FSH-LH - Prolactin Testosterone Test Overview

FSH-LH-Prolactin Testosterone test is a blood test to measure hormonal levels of FSH (Follicle stimulating hormone), LH (Luteinizing hormone), Prolactin hormone and Testosterone hormone in blood. All these four hormones are produced by the pituitary gland and are responsible for reproduction and sexual functions in males and females. 
FSH and LH are present both in men and women. In women it controls the menstrual cycle and stimulates the production of eggs (ovulation). In men it stimulates the production of sperm. It is present in low levels in children until puberty, post which the FSH levels increase to stimulate the production of oestrogen and testosterone.
Prolactin acts primarily on the mammary glands and its principal action involve - proliferation of mammary glands ; initiation of milk secretion . The levels of this hormone rises in pregnancy and during lactation. 
Testosterone is synthesized by both males and females. Male sex hormones (androgens) include testosterone.

The FSH-LH-Prolactin-Testosterone test is an assessment of a group of hormones responsible for reproduction and sexual functions in males and females. The tests analyze any hormonal changes which may cause infertility. The test may also check for damage or disease of the testes, ovaries, pituitary gland, or hypothalamus. It also evaluates fertility issues and menstrual problems.

4 hormones are tested in The FSH-LH-Prolactin-Testosterone test. They are;

Follicular Stimulating hormone (FSH), Luteinising Hormone (LH), Prolactin, and Testosterone in the blood.

Why is the FSH-LH-Prolactin-Testosterone test done?

These tests are used in different ways. This depends on whether you are a woman, man, or child. These tests are most often used:

  • To investigate the function of reproductive organs
  • To detect PCOS
  • To detect issues in conceiving for women.
  • To investigate infertility issues in males and females
  • To evaluate irregular menstrual cycle in women
  • To detect early or delayed sexual maturation in teens
  • To investigate low sperm count in men
  • To confirm the start of menopause, or perimenopause. Menopause is the end of the menstrual cycle and one cannot become pregnant.

What other tests are ordered along with the FSH-LH-Prolactin-Testosterone test?

Thyroid-stimulating hormone (TSH) is tested to know T3 and T4 hormone levels. Pregnancy also affects TSH levels. Thyroid disease can develop during pregnancy.

Written by: Dr.Shibani R, Medical Writer, Medical Affairs

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FSH LH Prolactin Testosterone Profile Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the FSH LH Prolactin Testosterone Profile Test with a clear pricing structure.

The FSH LH Prolactin Testosterone Profile Test Price in Mumbai is ₹ 1,970 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

Prolactin testosterone test is done to fertility issues in males and female. It may be done to diagnose menstrual irregularities and dysfunctions. In women having difficulties conceiving, it is done to diagnose the cause for infertility. Altered LH/FSH ratio may point towards PCOS. In lactating mothers the prolactin levels are assessed to diagnose problems in milk secretion. It is done in males to diagnose reasons for infertility, low sperm count or testicular issues. In children it may be done to diagnose early or delayed puberty based on the levels.

It helps in evaluating the function of reproductive organs in both males and females.

Prolactin testosterone test measures the levels of FSH, LH, Prolactin and Testosterone hormones in the blood.

The level of FSH-LH-Prolactin and Testosterone in blood.  FSH-LH-Prolactin is a hormone produced by the pituitary gland. It is a tiny gland that is found below the brain.

During prolactin testosterone test blood sample is taken from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. You may feel a  little sting when the needle goes in or out. This usually takes less than five minutes. In new-borns the blood is taken from the skin behind the heel.

An elastic band will be wrapped around the upper arm by a medical expert to exert pressure after the region of skin has been cleaned. You'll be instructed to make a fist. This allows blood to pass through the vein. The blood is then drawn out of the vein using a needle. The blood is drawn into a syringe or vial. To stop bleeding, the elastic band is unfastened and the area is wrapped with cotton or a bandage. The laboratory receives the blood sample for analysis.

FSH-LH-Prolactin needs to be present in certain levels at specific time of the menstrual cycle for successful conception. Abnormal levels in any of these may lead to PCOS and infertility issues. High FSH-LH levels in women may mean primary ovarian insufficiency, Turners syndrome, ovarian malignancies, or early onset of menopause. High LH/ FSH ratio is seen in polycystic ovarian syndrome (PCOS). 
In men, high FSH-LH may indicate testicular issues, Klinefelter syndrome or low sperm count. All these may indicate infertility in men. 
In children, high levels before puberty, signify early onset puberty. 
High prolactin levels may be seen in PCOS, hypothyroidism and hypogonadism. 
Low levels of FSH-LH may lead to lack of ovulation and sperm production leading to infertility issues in both men and women. Low levels may be due to problems in the hypothalamus or pituitary gland, being underweight or other central nervous system issues. 
In children, low FSH-LH levels may signify delayed puberty. 
High testosterone may occur in uterine fibroids in women and testicular tumours in men. Low levels may lead to lack of ovulation and sperm production leading to infertility issues in both men and women. Low levels may be due to problems in the hypothalamus or pituitary gland, being underweight or other central nervous system issues. In children, low levels may signify delayed puberty. It may be due to Turner's syndrome in females , Klinefelter's syndrome in males or some other infections causing sexual issues. Low prolactin levels may also occur due to pseudohypoparathyroidism.

The test is done for women and males to check fertility and hormonal imbalance.

Do not eat or drink (only drink water) for 8-12 hours before the test. In females, the sampling time is 2 or 3 days of the menstrual cycle.

  1. FSH and LH:
    Abnormal levels increase the risk of infertility, irregular periods, and delayed puberty. They also indicate primary ovarian insufficiency, ovarian malignancies, and testicular issues. Abnormal levels in males suggest a problem with the testicles.
  2. Prolactin:
    Abnormal levels indicate PCOS, hypothyroidism, and hypogonadism. High levels suggest non-cancerous tumours in men and women.
  3. Testosterone:
    A high level indicates acne, menstruation issues, PCOS and uterine fibroid in women and testicular tumours in men.

LH causes the testicles to make testosterone. This helps in the production of sperm. LH is very important for children. LH aids in directing the ovaries to produce oestrogen in females. It aids testosterone production in boys by the testes.

If there is an elevated level of FSH and LH and a low level of testosterone then it indicates Male Hypogonadism. Early detection can treat infertility due to hypogonadism.

  • In women- FSH is essential for the production of eggs by the ovaries. It also controls menstrual cycles.
  • In males, the role of FSH is to stimulate the testes to produce sperm.
  • In children- This rises before puberty begins and the development of secondary sexual characteristics.
  • In women- It stimulates the menstrual cycle. It peaks before ovulation.
  • In males- Testosterone production is dependent on LH. 

In females- It supports the growth of breasts. It increases milk production after childbirth.  •    In males- It affects sperm production.

  • In women- Combined with estrogen, testosterone helps with bone mass, moods, and supporting reproductive health. 
  • In males- It regulates libido, muscle mass, fertility, and sperm count.
  • In women- FSH is essential for the production of eggs by the ovaries. It also controls menstrual cycles.

In males, the role of FSH is to stimulate the testes to produce sperm.

In children- This rises before puberty begins and the development of secondary sexual characteristics.

Hormone Profile

Fertility Profile

Follicle Stimulating Hormone (FSH)

Luteinising Hormone (LH)

Prolactin (PRL)

Testosterone

full medical check up

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