ANCA by IFA reflex MPO/PR3 Antibodies Serum

The ANCA Reflex MPO/PR3 Antibodies test measures the presence and pattern of ANCA in the bloodstream. If positive, the levels of two specific types of ANCA, namely myeloperoxidase (MPO)-peripheral and proteinase 3 (PR3)-cytoplasmic are further detected (depending upon pattern as per international criteria). The pattern of fluorescence may be identified as cytoplasmic (cANCA), perinuclear (pANCA), or atypical ANCA (X-ANCA). The levels of MPO-ANCA and PR3-ANCA may be reported as negative, equivocal, or positive.

The ANCA Reflex MPO/PR3 Antibodies test measures the presence and pattern of ANCA in the bloodstream. If positive, the levels of two specific types of ANCA, namely myeloperoxidase (MPO)-peripheral and proteinase 3 (PR3)-cytoplasmic are further detected (depending upon pattern as per international criteria). The pattern of fluorescence may be identified as cytoplasmic (cANCA), perinuclear (pANCA), or atypical ANCA (X-ANCA). The levels of MPO-ANCA and PR3-ANCA may be reported as negative, equivocal, or positive.

• The ANCA Reflex MPO/PR3 Antibodies test is typically conducted to diagnose and monitor inflammatory activity in the small vessel vasculitides, which are a group of disorders associated with damage and weakening of blood vessels. These conditions can cause tissue and organ damage due to the narrowing and obstruction of blood vessels and the subsequent loss of blood supply. They can also produce areas of weakness in blood vessel walls, known as aneurysms, which have the potential to rupture. The symptoms experienced by a person with small vessel vasculitis depend upon the degree of autoimmune activity and the parts of the body involved.
• The test can also help differentiate between primary and secondary amyloidosis, as well as identify the source of amyloid deposits. Amyloidosis is a condition where abnormal proteins called amyloids accumulate in various organs and tissues, causing damage and dysfunction.

The ANCA reflex MPO/PR3 Antibodies Serum test may be recommended in the following situations:

• When a person presents with signs and symptoms of small vessel vasculitis, such as fever, fatigue, weight loss, joint pain, skin rash, nerve damage, or impaired kidney or lung function.
• When a person has a positive result for ANCA and needs further confirmation and specificity of the antibodies involved.
• When a person has a suspected or confirmed diagnosis of small vessel vasculitis and needs to monitor the disease activity and response to treatment.
• When a person has signs and symptoms of amyloidosis, such as swelling, bleeding, numbness, or organ failure.
• People experiencing symptoms or risk factors related to these conditions should discuss the test with their healthcare provider.

A positive result for cANCA and/or PR3-ANCA is highly specific for Wegener granulomatosis (WG), a form of small vessel vasculitis that affects mainly the upper and lower respiratory tract and the kidneys. A positive result for pANCA and/or MPO-ANCA is more common in microscopic polyangiitis (MPA) and its renal-limited variant (pauci-immune crescentic glomerulonephritis), which are forms of small vessel vasculitis that affect mainly the kidneys. A positive result for pANCA may also be seen in Churg-Strauss syndrome (CSS), a form of small vessel vasculitis that affects mainly the lungs and is associated with asthma and eosinophilia. A positive result for X-ANCA may be seen in some cases of primary or secondary amyloidosis.

The test typically involves a blood draw, where a healthcare professional will collect a small sample of blood from a vein, usually in the arm. The procedure is generally quick and less invasive.

You may be advised to follow specific instructions before the test, such as fasting for a certain period or discontinuing certain medications. It’s essential to follow your healthcare provider’s guidance to ensure accurate test results.

Additional tests that may be ordered alongside the ANCA reflex MPO/PR3 Antibodies test include:

• C-reactive protein (CRP) test: To measure another acute phase protein that indicates inflammation.
• Erythrocyte sedimentation rate (ESR) test: To measure how quickly red blood cells settle at the bottom of a tube, which reflects inflammation.
• Blood culture test: To detect any bacterial infection in the blood.
• Urine protein electrophoresis test: To identify any abnormal proteins in the urine that may indicate kidney damage or amyloidosis.
• Biopsy test: To obtain a tissue sample from an affected organ or site and examine it for vasculitis or amyloid deposits.

• ANCA with reflex to MPO/PR3
• ANCA Screen with MPO and PR3 Reflex
• ANCA with Reflex Testing for MPO & PR3

The anti-PR3 test is a blood test that detects antibodies against proteinase 3 (PR3), a specific enzyme found in certain white blood cells. It is primarily used to diagnose and monitor granulomatosis with polyangiitis (GPA) and other ANCA-associated vasculitides.

Metropolis Healthcare offers a convenient home collection service for the anti-PR3 test. A trained phlebotomist will visit your home to collect the blood sample, ensuring comfort, safety, and adherence to high standards of sample handling.

The anti-PR3 test is primarily used to diagnose granulomatosis with polyangiitis (GPA) and to differentiate it from other vasculitides. It is also used to monitor disease activity and response to treatment in patients with GPA.

The normal range for the anti-PR3 test is considered to be 19 AU/mL or less. Results between 20 and 25 AU/mL are considered equivocal, while results of 26 AU/mL or greater are considered positive.

Individuals with symptoms suggestive of ANCA-associated vasculitis, particularly granulomatosis with polyangiitis (GPA), are eligible for the anti-PR3 test. The test may also be recommended for patients with known GPA to monitor disease activity and treatment response.

The main benefits of the anti-PR3 test include its ability to aid in the diagnosis of GPA, differentiate GPA from other vasculitides, and monitor disease activity and treatment response in patients with GPA.

The frequency of anti-PR3 testing depends on individual circumstances, such as the presence of symptoms, diagnosis, and disease activity. Your healthcare provider will determine the appropriate testing schedule based on your specific situation.

There is no specific time requirement for the anti-PR3 test. The test can be performed at any time of the day, as it does not require fasting or other special preparations.

No, fasting is not required for the anti-PR3 test. You can have your regular meals and drinks before the test, as they do not affect the test results.

There are no specific precautions required before undergoing an anti-PR3 test. However, it is essential to inform your healthcare provider about any medications you are taking, as some drugs may influence the test results.

The main parameter included in the anti-PR3 test is the level of antibodies against proteinase 3 (PR3) in the blood. The test measures the concentration of these antibodies and provides results in AU/mL.

The anti-PR3 test is performed by collecting a blood sample from a vein in your arm. The sample is then sent to a laboratory, where it undergoes an antigen-specific immunoassay to detect the presence and concentration of PR3 antibodies.

The anti-PR3 test should be done when a person exhibits symptoms suggestive of ANCA-associated vasculitis, particularly granulomatosis with polyangiitis (GPA). It may also be performed periodically to monitor disease activity and treatment response in patients with known GPA.

The anti-pr3 test is considered positive when the level of PR3 antibodies in the blood is 26 AU/mL or greater. Positive results are highly specific for granulomatosis with polyangiitis (GPA) and indicate the presence of the disease.

The blood sample collection for the anti-PR3 test usually takes only a few minutes. However, the processing and analysis of the sample may take several days, depending on the laboratory and its workload.

The time required to receive the reports for the anti-PR3 test may vary depending on the lab and its procedures. Typically, results are available within 42 hours after the sample is collected.