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H1N1 (Swine Flu) RNA Detection By Real-Time PCR - Swab

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H1N1 Test Overview

H1N1 test detects influenza viruses. Influenza or flu is a viral infection which affects your respiratory system. Usually the infection is mild and resolves on its own but in children less than 5 years and adults older than 65 years or people with weakened immune systems or chronic diseases the infection can be life threatening. Symptoms are of fever, muscle pain, chills and sweats, headache, shortness of breath. 
Infection can be diagnosed with accuracy by performing a nucleic acid detection test i.e., PCR. However, this test should be done only if clinically required and it meets the epidemiological criteria

Real-time PCR is a powerful tool in the detection of H1N1 (swine flu) RNA. The process involves the use of a swab to collect a sample from the patient, which is then tested for the presence of the virus' genetic material through a polymerase chain reaction (PCR) technique. Real-time PCR is an effective method for quickly and accurately diagnosing infection with H1N1 because it can detect small amounts of the virus’ RNA within minutes. Furthermore, its sensitivity allows for the early identification of the virus before the onset of symptoms. To ensure accuracy, all samples are routinely retested using a variety of techniques, such as antigenic typing or sequencing. With the help of real-time PCR, H1N1 can be identified and treated rapidly, significantly reducing its potential spread.

What is Swine Flu?

Swine flu, also known as the H1N1 virus, is a contagious respiratory illness caused by a type of influenza virus that primarily affects pigs. The virus can spread from person to person and can cause mild to severe illness. Symptoms of swine flu include fever, cough, sore throat, body aches, headache, chills, fatigue, and sometimes vomiting and diarrhea. Swine flu can be serious, even deadly, especially in people with chronic health problems and compromised immune systems.

Who should get H1N1 swine flu RNA detection by real-time PCR swab test done?

  • People who have had recent contact with someone infected by H1N1 swine flu.
  • People who have traveled to an area known to have cases of H1N1 swine flu.
  • People who have symptoms of H1N1 swine flu, such as fever, cough, sore throat, and body aches.
  • People at higher risk of complications from H1N1 swine flu, such as pregnant women, individuals with chronic health conditions, and young children.
  • Healthcare workers who have been in close contact with patients known to be infected with H1N1 swine flu.

Written by: Dr. Vishal Wadhwa, M.D, D.N.B Microbiology, Medical Affairs

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H1N1 (Swine Flu) RNA Detection By Real-Time PCR - Swab Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the H1N1 (Swine Flu) RNA Detection By Real-Time PCR - Swab with a clear pricing structure.

The H1N1 (Swine Flu) RNA Detection By Real-Time PCR - Swab Price in Mumbai is ₹ 5,000 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

H1N1 test is used to diagnose H1N1 infection when clinical and epidemilogical criteria suggest H1N1 infection possibility

H1N1 swine flu RNA detection by real-time PCR swab test is done to accurately diagnose the presence of the H1N1 swine flu virus in a patient. This test can detect the genetic material of the virus within minutes and helps to quickly diagnose and provide treatment. It also helps to identify which strain of the virus is present, which is important for determining the best course of treatment.

  • To determine whether an individual has been infected with the H1N1 swine flu virus.
  • To identify the presence of virus-specific RNA sequences in a sample from an infected person.
  • To help doctors determine how to best treat an infected patient.
  • To monitor the spread of the virus in the population.
  • To detect the virus early and prevent further transmission.
  • To provide timely information for public health authorities to plan and implement containment strategies.
  • To develop new methods of antiviral therapy.
  • To create better vaccines against the virus.

H1N1 test detects RNA of Influenza A, Influenza A/ H1N1 virus

A nasopharyngeal and a throat swab will be collected by our trained phlebotomist, who will wear necessary preventive barriers before collection. Instructions will be given to you on what to expect before sample collection. In brief, one swab will be introduced through the nose to collect sample from the nasopharynx and a second swab will be used to collect sample from the throat. Process usually takes less than 5 minutes post all preparations have been done.

H1N1 detected result means that virus RNA has been detected in sample and patient is likely to be suffering from H1N1 infection. It is important to note that this detect will detect RNA of dead virus and hence clinical correlation should be done to interpret the results
H1n1 not detected result means that virus RNA has not been detected in the patient sample. Timing of samples collection is important. Virus can be detected during 1st week of illness

  1. There are no significant risks or complications associated with this test.

The following tests are commonly ordered along with the real-time PCR swab test for precise diagnosis:

  • Complete Blood Count (CBC).
  • Chest X-ray.
  • Influenza A and B Antibody Test.
  • Serum Immunoglobulins Test.
  • Viral Culture Test.
  • Rapid Influenza Diagnostic Test (RIDT).

RIDT is a quick and easy way to diagnose influenza. It involves collecting a sample of mucus or saliva from the nose or throat and testing it for the presence of the influenza virus. Results are typically available within minutes, allowing healthcare providers to quickly identify and treat the infection before it spreads.

Influenza A virus

Pandemic Swine Influenza A virus

Pandemic Swine H1 influenza virus


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