Vitamin Profile Test
Vitamin Profile Test Overview
Vitamin D is crucial for the absorption of calcium, and a lack of it can cause discomfort, weak bones, and irregular bone growth. Additionally, it aids in keeping parathyroid hormone, calcium, and phosphate levels in check. PTH, a parathyroid hormone, regulates blood calcium levels. The vitamin profile test is frequently carried out to determine the cause of osteoporosis, kidney stones, hyper- or hypocalcemia, and low vitamin D levels. The purpose of the vitamin profile is to evaluate the condition of other nutrients, such as vitamin B12, calcium, and magnesium, which are frequently impacted when levels are aberrant.
Created by: Dr.Shibani R Medical Writer, Medical Affairs
Reasons for Undergoing the Vitamin Profile Test
There are several compelling reasons to undergo a vitamin profile test:
- If you are experiencing symptoms that could indicate a vitamin deficiency, such as fatigue, muscle weakness, bone pain, or neurological issues, your doctor may recommend a vitamin profile test.
- Individuals with certain conditions or lifestyles that increase the risk of vitamin deficiencies, such as celiac or Crohn's disease, malabsorption syndrome, alcohol use disorder, limited sun exposure, darker skin pigmentation, obesity, or a plant-based diet (vegetarian or vegan), may need a vitamin profile test.
- To check the effectiveness of vitamin supplements or to monitor vitamin levels in individuals who are already taking supplements
- As part of a routine health screening to identify potential nutritional deficiencies before they lead to serious health issues
Preparation For Vitamin Profile Test
Getting ready for your Vitamin Profile test is simple and stress-free. You don’t need to worry about any complicated steps, but being informed helps make the experience smoother.
Here are a few things to keep in mind for yourVitamin Profile test preparation:
- No fasting is required: You can eat and drink normally before the test unless your doctor advises otherwise.
- Inform your doctor about medications: Certain supplements or medicines might affect vitamin levels, so always let your healthcare provider know what you're taking.
- Stay hydrated: Drink water before the test, as it can make the blood draw easier.
- Wear comfortable clothing: Especially one that allows easy access to your arm for the blood sample.
- Stay calm: The test is routine and usually completed within minutes. Being relaxed helps ease the process.
Procedure of the Vitamin Profile Test
The Vitamin Profile test sample collection procedure is straightforward and typically completed within a few minutes. It is designed to be as comfortable and convenient as possible.
Here is how it works:
- Sample collection: A trained technician draws a small amount of blood from a vein in your arm.
- No discomfort expected: You may feel a brief pinch, but it's usually painless.
- Home collection option: Many diagnostic labs like Metropolis Healthcare offer home sample collection to make things even easier.
- Sample handling: The blood is securely transported to the lab where it’s analysed for key vitamins like D, B12, and others.
- Report delivery: Results are often available within 24 hours and shared digitally or in person.
The whole process is safe, quick, and conducted using hygienic, certified methods.
Symptoms That May Call For Vitamin Profile Test
Sometimes, your body gives subtle signals that could point towards vitamin deficiencies. A Vitamin Profile test can help identify if something is off and offer timely insights.
Here are common symptoms that might suggest the need for this test:
- Persistent fatigue or tiredness
- Muscle weakness or cramps
- Bone pain or softening
- Mood changes or irritability
- Numbness or tingling in limbs
- Poor wound healing or frequent infections
If you notice any of these, don’t panic—but do speak with your doctor and consider a Vitamin Profile test for clarity.
Vitamin Profile Test Interpretation & Clinical utility :
Vitamin Profile test is a combination of test comprising of Vitamins, hormone & minerals which are commonest nutritional deficiency & are involved in metabolic disorders. This test helps in dietary planning & targeted supplementation. The test parameters are –
Ionised Calcium : Ionised calcium is the preferred measurement rather than total calcium because it is physiologically active and can be rapidly measured. Useful in diagnosing patients with hypocalcemia or hypercalcemia with borderline serum calcium and altered serum proteins.
Low levels are seen commonly in primary hypoparathyroidism, vitamin D deficiency, renal disorders, malabsorption, Osteomalacia, Pancreatitis, and rickets
High levels are seen in primary hyperparathyroidism, vitamin D therapy, various malignancies producing parathyroid hormone, Acidosis, Metastatic bone tumor, Milk-alkali syndrome, Multiple Myeloma Paget disease, and Sarcoidosis.
Magnesium :
Magnesium is a mineral that is important for immunity, muscle contraction, nerve function, strong bones and regulating blood pressure as well as blood sugar.
High levels of magnesium may result from taking too many supplements/antacids/laxatives that contain magnesium, kidney disease, Addison disease, dehydration and diabetic ketoacidosis. Low levels of magnesium may be seen in chronic diarrhea, hemodialysis, gastrointestinal disorders, cirrhosis, hyperaldosteronism, hypoparathyroidism, severe burns, pancreatitis, preeclampsia, ulcerative colitis and uncontrolled diabetes
Vitamin D & Parathyroid hormone (PTH) :
Vitamin D and PTH play crucial roles in calcium and phosphate homeostasis, and their interplay is essential for bone health. Vitamin D regulate calcium absorption in the intestines and bone resorption, increasing calcium and phosphate levels in the blood. PTH, secreted by the parathyroid glands, also regulates calcium and phosphate levels. For diagnosis of vitamin D deficiency it is recommended to have clinical correlation with serum vitamin D, serum calcium & serum PTH. An inverse relationship exists between PTH and Vitamin D levels.
Holotranscobalamin (Active Vitamin B12) :
This test helps evaluate the functional, active form of vitamin B12, which is crucial for proper bodily functions. It specifically measures the vitamin B12 that is actively transported into cells, making it a key indicator of B12 availability. Unlike total vitamin B12 tests, which measure all forms of Vitamin B12, the Holotranscobalamin test provides a more direct measure of the B12 that is actually being used by the body. Low levels can indicate a deficiency, even if total B12 levels are within the normal range. The test can be used to track the effectiveness of B12 supplementation or treatment for deficiency.
Always consult your healthcare provider to understand your Vitamin Profile test result in context of your overall health.
Factors That Can Affect the Vitamin Profile Test Result
Several external and internal factors can influence the outcome of your Vitamin Profile test. Being aware of them ensures you get the most accurate and meaningful insights.
Here is what might affect your results:
- Recent supplements or multivitamins: These can temporarily alter vitamin levels in your blood.
- Medications: Some drugs can interfere with how your body absorbs or processes vitamins.
- Underlying medical conditions: Conditions like Crohn’s disease or kidney disorders may impact vitamin absorption.
- Lifestyle factors: Smoking, alcohol use, or a highly restrictive diet can skew results.
- Time of year and sun exposure: Vitamin D levels are affected by seasonal changes and how much sunlight you receive.
If you are concerned about anything possibly affecting yourVitamin Profile test result, speak with your doctor for tailored advice.
Conclusion
Staying ahead of your health starts with understanding what your body needs. A Vitamin Profile test helps detect deficiencies early, so you can take the right steps with confidence. For accurate reports, expert support, and home collection convenience, trust Metropolis Healthcare—a leader in diagnostics with 40+ years of experience and NABL & CAP-accredited labs nationwide.
List of Parameters Considered During the Vitamin Profile Test
During a vitamin profile test, several key parameters are measured:
- Vitamin D: This vitamin is crucial for bone health, muscle function, and immune system integrity. The vitamin profile test measures 25-hydroxyvitamin D (25(OH)D) levels, which indicate the body's vitamin D status. Low levels can suggest deficiencies, malabsorption, or kidney/liver issues.
- Vitamin B12: This vitamin is essential for energy metabolism, blood cell formation, and nervous system function. Deficiencies can lead to fatigue, neurological problems, and certain types of anaemia.
- Other B Vitamins: Depending on the test, other B vitamins such as thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxal phosphate (B6), biotin (B7), and folate (B9) may be measured. Each B vitamin has distinct roles in metabolism, blood cell production, and nervous system health.
- Vitamin E: Though less commonly included, vitamin E is important for antioxidant functions and overall health. Deficiencies can lead to neurological problems and impaired immune function.
Home Collection for Vitamin Profile Test Near You
Choosing Metropolis Healthcare's home collection service for your vitamin profile test comes with several advantages. A trained phlebotomist will visit your home at a time that suits you, ensuring both comfort and safety while collecting your blood sample.
Metropolis Healthcare maintains high standards of sample handling protocols, ensuring that the accuracy of your vitamin profile test is never compromised. You can expect to receive timely and accurate results, as Metropolis Healthcare is dedicated to enhancing healthcare accessibility and providing high-quality diagnostic solutions directly at your doorstep.
Vitamin Profile Test Price
Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Vitamin Profile Test with a clear pricing structure.
The Vitamin Profile Test Price in Mumbai is ₹ 3,120 .
We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.
Frequently Asked Questions
- Low vitamin D levels can help diagnose a case of secondary hyperparathyroidism.
- PTH test is most often used along with vitamin D levels to diagnose hypoparathyroidism & monitor kidney disease.
- It is also used to diagnose cause for osteoporosis & kidney stones.
- Vitamin B12, calcium and magnesium levels are also important for efficient functioning of nerves, muscles and even cardiac health.
Doctors frequently prescribe a vitamin profile test for patients exhibiting any of the below-listed symptoms that cannot be adequately diagnosed or treated:
- Anorexia or significant weight loss.
- Hair loss.
- Fatigue.
- Inability to stand up straight after eating.
- Bone pain brought on by cytokine-mediated inflammation (increased CRP).
- Elevated liver enzymes (such as AST/ALT) with normal bilirubin levels.
- Ultrasound-confirmed hepatomegaly, or an enlarged liver.
In addition to the vitamin profile test, a few more tests like the following will also be ordered:
- TSH.
- Thyroid-stimulating hormone.
- Ionized calcium.
- Magnesium.
- Folic acid or folates.
- Vitamin A.
- Vitamin profile test is used to diagnose vitamin D, vitamin B12 and micronutrient deficiency in the blood.
- It measures 25 hydroxy vitamin D levels. If the levels are abnormal, PTH levels in blood are tested along with active vitamin B12, calcium and magnesium levels.
The vitamin profile test is a comprehensive blood test that measures the levels of different vitamins in the body, particularly vitamins D, B12, and occasionally other B vitamins and vitamin E. It is helps assess nutritional status, identify deficiencies, and evaluate the effectiveness of vitamin supplements.
The vitamin profile test is vital for the early detection of vitamin deficiencies, which can result in serious health complications like bone disorders (osteoporosis, rickets), neurological issues, and metabolic imbalances.
By identifying deficiencies early, it allows for timely intervention and preventive measures, helping to safeguard overall health and well-being.
The vitamin profile test detects the levels of essential vitamins in the blood, with a primary focus on vitamins D and B12, and sometimes other B vitamins and vitamin E. It detects deficiencies may be caused by insufficient dietary intake, malabsorption, or underlying health conditions.
The vitamin profile test is also useful in diagnosing disorders like osteomalacia, anemia, and neurological issues linked to vitamin deficiencies. By assessing these vital vitamin levels, healthcare providers can assess an individual's nutritional status and determine if corrective actions, such as dietary adjustments or supplements, are needed to support optimal health.
Vitamin profile test reports indicate the levels of various vitamins in the blood. If the levels are normal, it suggests adequate nutritional status. However, if the levels are abnormal, it may indicate a deficiency or insufficiency. For instance, low vitamin D levels could suggest limited sun exposure, dietary insufficiency, or malabsorption issues. Abnormal levels may prompt further tests to determine the underlying cause and guide the need for supplements or other treatments. Your healthcare provider will interpret the results in the context of your medical history and symptoms. They will consider factors such as age, gender, lifestyle, and any pre-existing medical conditions to provide a comprehensive assessment of your nutritional status and recommend appropriate interventions if necessary.
If the vitamin profile test reports indicate a deficiency or insufficiency, your healthcare provider may recommend vitamin supplements or dietary changes to correct the imbalance. In some cases, additional tests might be ordered to determine the cause of the deficiency, such as a parathyroid panel test for low vitamin D levels. The provider may also suggest intravenous treatment if necessary. The goal is to restore optimal vitamin levels and prevent any potential health complications associated with the deficiency.
The vitamin profile test offers several key benefits, including an early detection of vitamin deficiencies, which can help prevent serious health complications. It helps in monitoring the effectiveness of vitamin supplements and provides valuable insights for making dietary adjustments to achieve optimal nutritional balance.
Furthermore, a vitamin profile test provides a comprehensive assessment of the individual’s nutritional status, empowering them to make an informed decisions to enhance their overall health and well-being.
There are no significant risks associated with the vitamin profile test. It is a simple blood test that involves minimal discomfort, similar to any other blood test.
The vitamin profile test can help diagnose diseases related to vitamin deficiencies, such as:
- Osteomalacia and osteoporosis due to vitamin D deficiency
- Anaemia and neurological disorders due to vitamin B12 deficiency
- Rickets in children due to vitamin D deficiency
- Malabsorption syndromes and digestive disorders related to B vitamin deficiencies
Yes, pregnant women can undergo a vitamin profile test, especially since pregnancy increases the demand for certain vitamins. It is crucial for monitoring and ensuring adequate nutritional levels to support both maternal and foetal health.
Yes, certain medications can affect the results of your vitamin profile test. It is important to inform your healthcare provider about any medications you are taking before the test to ensure accurate results.
No, fasting is not necessary before taking the vitamin profile test. However, it is advisable to consult your doctor before taking any over-the-counter medications to ensure the accuracy of the test results.
Several factors can affect your vitamin profile results, including an inflammation, certain medications, and kidney or liver disease. Different laboratories may also have varying reference ranges for normal levels, which can influence the interpretation of the results.
Vitamin profile test results are typically available after 8 hours, though this can vary depending on the healthcare provider and the time or day the sample is collected and processed.
You can easily book online or call Metropolis Healthcare for appointment and confirmation.
Your test results will be shared via email, SMS, or app within 24 hours.
Yes, services like Metropolis Healthcare offer convenient home collection service by trained phlebotomists at your chosen time.
Ratings & Reviews (0)
Why Metropolis?
Metropolis has a team of 200 senior pathologists and over 2000 technicians delivering diagnostic solutions in the areas of routine, semi specialty and super specialty domains like Oncology, Neurology, Gynaecology, Nephrology and many more.
We offer a comprehensive range of 4000+ clinical laboratory tests and profiles, which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease.

WhatsApp