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TPO (Thyroid Peroxidase) Antibody (AMA) Microsomal Antibody Serum Test

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TPO Antibody Test Overview

Thyroperoxidase (TPO) is an enzyme involved in thyroid hormone synthesis. 
Thyroid peroxidase antibodies (TPO) antibodies destroy cells of thyroid gland and cause hypothyroidism. These antibodies are hallmark of Hashimoto thyroiditis but can be seen in Graves' disease also.

Thyroid-related disorders are a common occurrence these days and occur due to malfunction of the thyroid gland. Thyroid peroxidase antibody testing is carried out when it is believed that an autoimmune condition is the root of a thyroid issue. When your immune system unintentionally targets the thyroid gland or its protein constituents, thyroid antibodies form and can result in tissue harm, chronic thyroid inflammation, and/or impairment of thyroid function. Here is all you need to know about the TPO antibody test

Written by: Dr Vishal Wadhwa, M.D, D.N.B Microbiology, Medical Affairs

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TPO (Thyroid Peroxidase) Antibody (AMA) Microsomal Antibody Serum Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the TPO (Thyroid Peroxidase) Antibody (AMA) Microsomal Antibody Serum Test with a clear pricing structure.

The TPO (Thyroid Peroxidase) Antibody (AMA) Microsomal Antibody Serum Test Price in Mumbai is ₹ 1,330 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

TPO antibody test is used to aid diagnosis of autoimmune disorder as cause of hypothyroidism

Testing for thyroid antibodies is used to determine whether a patient has autoimmune thyroid disease-related hypothyroidism or hyperthyroidism. An underactive thyroid gland leads to hypothyroidism, and a hyperactive thyroid gland leads to hyperthyroidism. Additional uses for thyroid antibody testing include the ones listed below -

  1. To identify autoimmune diseases like Graves' disease or Hashimoto's thyroiditis.
  2. Choosing the most effective line of action.
  3. To test the likelihood that your mild hypothyroidism will worsen over time.
  4. To assess the fetal risk in pregnant women with thyroid disease.
  5. To calculate the probability of relapse during Graves' disease therapy.
  6. To check if you have ever received treatment for thyroid cancer.

TPO antibody test measures the level of anti TPO antibody in blood

The TPO antibody test measures the levels of thyroid peroxidase antibodies that are targeted against the enzyme thyroid peroxidase. The test helps to differentiate between thyroiditis and hypothyroidism. Thyroiditis is an inflammation of the thyroid gland that usually occurs due to immune system disorders such as Hashimoto's thyroiditis.

TPO antibody test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer.


Normal levels : no thyroid antibodies were found. This means that symptoms of hypothyroidism are probably not caused by decrease in TPO.
High values: suggestive of  Hashimoto thyroiditis; these antibodies can also be seen in Grave's disease

You could require this test if you experience thyroid-related symptoms and your doctor thinks Hashimoto's or Grave's disease is to blame.

You might also need this test if results from previous thyroid tests indicate that your thyroid hormone levels are abnormally high or low. These tests include hormone assays for T3, T4, and TSH (thyroid-stimulating hormone).

To assist in identifying Hashimoto's thyroiditis, the TPO antibody test is used. If you experience any of the following symptoms, consider getting tested -

  1. Fatigue.
  2. Irregular menstrual periods.
  3. Arid skin.
  4. Broken nails.
  5. Constipation.
  6. Cold sensitivity.
  7. Aches and pains in muscles.
  8. Depression.
  9. Mental haze.

The normal range of thyroid peroxidase antibodies is 9.0 IU/mL. All ages are included in the reference values.

Increased thyroid peroxidase antibody levels are frequently linked to thyroid issues, which are recognised to be a contributing factor in infertility.

Graves disease is a disorder of the immune system which leads to hyperthyroidism due to the excess production of thyroid hormones. This can cause bulging of the eyes and goitre (enlargement of the thyroid gland)

Hashimoto's thyroiditis is an autoimmune disease where the immune cells in the body mistakenly attack the cells in the thyroid gland that produce thyroid hormones. This eventually leads to hypothyroidism.

Thyroperoxidase (TPO) is a thyroid hormone-producing enzyme. In certain autoimmune conditions, antibodies against this enzyme are produced by the immune cells and are called TPO antibodies. These antibodies target and destroy the enzymes leading to hypothyroidism. TPO antibodies can be found

Your doctor may also recommend additional thyroid tests, such as tests for,

  • Thyroglobulin antibodies.
  • Thyroid-stimulating hormone (TSH).
  • Free T4 hormone.
  • T3 hormone.

If you have thyroid-related symptoms and your doctor suspects Hashimoto's or Grave's disease, you could need this test.

If other thyroid tests, such as T3, T4, and TSH (thyroid-stimulating hormone) hormone assays reveal that the thyroid hormone levels are abnormally high or low, you could also require this test.

  • Thyroid autoantibodies.
  • Thyroid peroxidase antibody.
  • TPO Test
  • Anti-TPO antibodies
  • Thyroperoxidase (TPO)
  • Iodide peroxidase
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