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PAP smear by Liquid based cytology (LBC) Test

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LBC Test Overview

LBC test performed for the detection of cervical cancer as well as HPV in women. It also detects early precancerous changes in the cells and hence with timely intervention progression to cancer can be prevented. The cells are collected from the cervix (lower end of vagina). Early detection helps in better diagnosis and prognosis. It is recommended between 25 years of age up to 65 years. As per (ACS/ASCCP/ASCP) tests should be done every 3 years. The frequency depends on the previous gynaecological history, risk factors and the tests undertaken. This is a less invasive test giving better results. It is preferred over the conventional method. 

Pap smear is a test to detect the risk of cervical cancer and to study the changes in cells during early-stage cervical cancer. This simple test involves a gynaecologist collecting cells from the cervix (the lower and narrow end of the uterus) and a pathology team analysing the cells. As opposed to a conventional pap smear test, the pap smear liquid-based cytology test is more precise and has fewer instances of giving a false-positive result.

Why is the Pap smear liquid-based cytology test done?

  • Pap smear liquid-based cytology test helps in knowing the risk of cervical cancer and observing changes in the cells in early-stage cancer.
  • This test helps catch cancer early or know if you are at a risk of developing cervical cancer and take measures to treat or prevent it at the earliest.

Written by: Dr.Shibani R, Medical Writer, Medical Affairs

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PAP smear by Liquid based cytology (LBC) Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the PAP smear by Liquid based cytology (LBC) Test with a clear pricing structure.

The PAP smear by Liquid based cytology (LBC) Test Price in Mumbai is ₹ 1,350 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

LBC Test is done for detection of cervical cancer and early cancerous changes in the cervix. It also additionally detects any infections of cervix. The test is usually done between 25 to 65 years every three or five years as per the doctor's recommendation. It may be done as a routine test along with the pelvic exam or is also done in certain high risk cases. It is often combined with HPV (Human papilloma virus ) testing which is more effective in detecting early changes of cancer since HPV infection is one of the major risk factor linked to cervical cancer. Other risk factor include:

  • History of previous cervical infection or precancerous cells in previous exam.
  • History of sexually transmitted infections like HIV.
  • Weakened immunity such as auto-immune disorder or previous organ transplant.
  • Exposure to DES (Diethylstilbestrol) prior to birth.

LBC test measures the amount of precancerous cells in the cervix (lower end of the vagina) as well as HPV.

Pap smear is usually performed at the doctor's clinic. A speculum is inserted into the vagina for a cervical examination, and using a spatula or soft brush the cervical cells are collected. The cells are dissolved in a liquid (LBC) for better results. 

Mostly in general population with low risk factors the test is negative. Normal or negative result rules out risk for cervical cancer. Next screening may be recommended after 3-5 years depending on your age and clinical history. In cases of infections and mild abnormal changes, strict vigilance, proper infection control and follow up after 3-5 years is required as changes tend to regress spontaneously. PAP smear with LBC reduces the chances of false positive. Hence, preferred over the conventional method. Major or severe abnormalities have increased risk of cancer progression and require early and timely intervention. Regular Pap smear test thus helps in early diagnosis and better prognosis.

Negative result: A negative result means that you are not at risk of developing cervical cancer at least for a few years.

Positive result: A positive result could mean that there are changes in your cervical cells, which could be due to several reasons:

  • Inflammation due to intercourse or usage of diaphragms
  • HPV or other infection
  • Pre-cancer or cancer
  • False positive

Usually, after a positive result, your gynaecologist may ask you to undergo an HPV DNA test to look for the presence of the HPV and the likelihood of cervical cancer.

No, the test is not painful. Your doctor uses a speculum to widen the opening of the vagina and uses a cell scraper to scrape some cells of the cervix.

The cells are stored in a vial and are taken to a pathologist. The pathologist examines the cells under a microscope and looks for abnormalities in shape, structure and organisation.

Yes. Your doctor may ask you to refrain from having sex or using tampons, ointments or creams at least 48 hours before the test. You may also be asked to avoid taking some medication that may bring about changes in the cervix.

No. The test is taken only away from your menstruation days in order to obtain the most appropriate results.

No. It does not mean that you have cervical cancer. Your doctor has suggested the test to look for infections and inflammation or to look for changes in the cervix, which may or may not be due to cervical cancer.

No, it is a non-invasive test. Even though the test is performed intravaginally, there is no cut involved.

For the conventional type of PAP smear test, the cells collected with a brush and a spatula are smeared on a slide and stained with PAP stain. For the liquid-based cytology type, cells collected brush are thoroughly rinsed in a preservation vial.

A pap smear liquid-based cytology test is similar to the conventional test in the procedure, the only difference is the method opted to analyse the cells under a microscope. Your gynaecologist inserts a speculum through your vagina to widen the opening and scrape a few cells from your cervix. The collected cells will then be stored in a vial and given to the pathology team for analysis under a microscope.

You may be asked to refrain from having sex or using tampons, creams, powders, contraceptives, suppositories or anything that could interfere with the cells in the cervix for at least 48 hours prior to the test.

A pap smear test is a common procedure that every woman of reproductive age (25 to 55 years) should undergo once every 3 to 5 years.

You may be asked to undergo a pap smear test more often if you:

  • Show signs of cervical cancer
  • Have early-stage cervical cancer
  • Have HIV infection
  • Have a family history of cervical cancer
  • Have auto-immune disorders

Since this test only gives information on the changes occurring in the cells, it is paired often with a Human Papilloma Virus (HPV) test to detect the presence of the HPV that causes cervical cancer in the cervix.

Other tests that may be ordered along with a Pap smear liquid-based cytology test include:

  • A pelvic examination to check for any abnormalities in the reproductive organs
  • A mammogram to check for any changes in the breast tissue
  • Thinprep test
  • Liquid-based cytology test
  • LBC test
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