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FSH LH Prolactin Serum Test

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FSH LH Prolactin Serum Test Overview

FSH-LH-Prolactin Serum test is a blood test to measure hormonal levels of FSH (Follicle stimulating hormone), LH (Luteinizing hormone) and Prolactin in blood. All these three hormones are produced by the pituitary gland and are responsible for reproduction and sexual functions in males and females. 
FSH and LH are present both in men and women. In women it controls the menstrual cycle and stimulates the production of eggs(ovulation). In men it stimulates the production of sperm. It is present in low levels in children until puberty, post which the FSH levels increase to stimulate the production of oestrogen and testosterone.
Prolactin acts primarily on the mammary glands and its principal action involve - proliferation of mammary glands ; initiation of milk secretion . The levels of this hormone rises in pregnancy and during lactation.


The FSH-LH-Prolactin Serum test is used to measure the amounts of certain hormones in the blood. FSH stands for Follicle Stimulating Hormone, and LH for Luteinising Hormone. FSH in women is especially active during the first two weeks of the period cycle as it makes the ovaries produce increased amounts of estrogen and some progesterone. FSH maintains normal sperm production in men. LH has similar roles in the fertility function in both men and women. Prolactin helps in milk production in women after childbirth. A trace amount of prolactin is also present in men.

All of these hormones are secreted by the pituitary gland in the brain. The hormones FSH and LH are present in both men and women. In women, it helps maintain the menstrual cycle and plays a role in ovulation. In men, FSH helps in maintaining normal sperm production. These hormones are present in low levels in children till they hit puberty.

Prolactin is present in women and it stimulates milk production from the mammary glands. Prolactin levels increase especially during pregnancy and lactation.

Why is the FSH-LH-Prolactin serum test done?

  • This test is done to check for fertility issues in both men and women.
  • In women, it is done at various ages. If facing menstrual irregularities, this test is done to diagnose the reasons behind it.
  • In women of reproductive age, this test is done to diagnose issues in conceiving.
  • In males, it is mainly done to examine infertility, reduced sperm count, testicular issues or thyroid disorders.
  • In children, these tests are done to diagnose early or delayed puberty.

If you have the following issues, your doctor will suggest this test:

  • Difficulty conceiving
  • Infertility in men and women
  • Low sperm count
  • Irregular periods / Poly Cystic Ovary Syndrome(PCOS) / Polycystic Ovarian Disease (PCOD)
  • Sudden weight gain
  • Early puberty
  • Late puberty
  • Reduced lactation after delivery

Written by: Dr.Shibani R, Medical Writer, Medical Affairs

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FSH LH Prolactin Serum Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the FSH LH Prolactin Serum Test with a clear pricing structure.

The FSH LH Prolactin Serum Test Price in Mumbai is ₹ 1,650 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

fsh lh prolactin serum test is done to fertility issues in males and female. It may be done to diagnose menstrual irregularities and dysfunctions. In women having difficulties conceiving, it is done to diagnose the cause for infertility. Altered LH/FSH ratio may point towards PCOS. In lactating mothers the prolactin levels are assessed to diagnose problems in milk secretion. It is done in males to diagnose reasons for infertility, low sperm count or testicular issues. In children it may be done to diagnose early or delayed puberty based on the levels.

fsh lh prolactin serum test measures the levels of FSH, LH and Prolactin hormones in the blood.

During fsh lh prolactin serum test blood sample is taken from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. You may feel a  little sting when the needle goes in or out. This usually takes less than five minutes.

The blood sample is drawn from your arm. An elastic band is tied to the upper arm. Then, you will be told to form a fist with your hand for faster blood flow and ease of drawing blood. An antiseptic is applied on your skin and then the injection is inserted and the blood is drawn. The blood is collected in a vacutainer.

FSH-LH-Prolactin needs to be present in certain levels at specific time of the menstrual cycle for successful conception. Abnormal levels in any of these may lead to PCOS and infertility issues. High FSH-LH levels in women may mean primary ovarian insufficiency, Turners syndrome, ovarian malignancies, or early onset of menopause. High LH/ FSH ratio is seen in polycystic ovarian syndrome (PCOS). 
In men, high FSH-LH may indicate testicular issues, Klinefelter syndrome or low sperm count. All these may indicate infertility in men. 
In children, high levels before puberty, signify early onset puberty. 
High prolactin levels may be seen in PCOS, hypothyroidism and hypogonadism. 
Low levels of FSH-LH may lead to lack of ovulation and sperm production leading to infertility issues in both men and women. Low levels may be due to problems in the hypothalamus or pituitary gland, being underweight or other central nervous system issues. 
In children, low FSH-LH levels may signify delayed puberty.

Wear loose-fitting clothes. Ask the doctor about any doubts you may have. Drink only water for 8-12 hours before the test. The FSH and LH are best taken on the 2nd or 3rd day of the menstrual cycle.

The reference range for follicle-stimulating hormone (FSH) is as follows:


Follicular phase: 3.1-7.9 mIU/mL

Ovulation peak: 2.3-18.5 mIU/mL

Luteal phase: 1.4-5.5 mIU/mL

Postmenopausal: 30.6-106.3 mIU/mL


Age 0-7 years: <6.7 mIU/mL

Age 8 years-adult:1.3-19.3 mIU/mL

The following values are considered to be normal LH blood levels:


Follicular phase of the menstrual cycle: 1.9 to 12.5 IU/L

LH surge: 8.7 to 76.3 IU/L

Luteal phase of the menstrual cycle: 0.5 to 16.9 IU/L

Pregnancy: Less than 1.5 IU/L

Post-menopause: 15.9 to 54.0 IU/L

Women using contraceptives: 0.7 to 5.6 IU/L


The age group of 20 and 70 years: 0.7 to 7.9 IU/L

>70 years of age: 3.1 to 34.0 IU/L

The following values are considered to be normal prolactin blood levels:

Males > 18 years: 2.10 - 17.70 ng/mL

Adult Females

Non-pregnant: 2.80 - 29.20 ng/mL

Pregnant: 9.70 - 208.50 ng/mL

Post Menopausal: 1.80 - 20.30 ng/mL

Some of the tests that are ordered along with the FSH-LH-Prolactin serum test are as follows:

  • Anti-Mullerian Hormone (AMH) test
  • Fertility Panel
  • Hormone Profile
  • Fertility Profile
  • Follicle Stimulating Hormone (FSH)
  • Luteinising Hormone (LH)
  • Prolactin (PRL)
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