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HCV-Hepatitis C RNA Detection by Taqman

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HCV RNA Test Overview

HCV infection is one of the most common causes of chronic liver disease, accounting for 60-70% of all chronic hepatitis. 
Acute HCV infection is often asymptomatic, or associated with non-specific symptoms, and usually goes undiagnosed. However, 60% to 85% of patients develop chronic infection, which is associated with increased risk of cirrhosis, end-stage liver disease, and hepatocellular carcinoma.
Detection and quantitation of HCV RNA is an important component of diagnosis and treatment monitoring

Hepatitis C Virus infection is one of the most common causes of chronic liver disease, accounting for 60 to 70% of all chronic hepatitis. Hepatitis C virus (HCV), a positive-strand RNA virus, is still a major health problem worldwide.

It frequently leads to chronic infection contributing to liver cirrhosis and hepatocellular carcinoma. Based on HCV genotype, antiviral therapy is successful in approximately 50 to 70% of infected patients

The HCV RNA PCR test is conducted through a process called polymerase chain reaction (PCR). There are two approaches to this process: qualitative and quantitative. The qualitative test just confirms the detection of the virus whether it is present or absent. On the other hand, the quantitative test gives the amount of virus present in the serum or plasma.

Written by: Dr. Niranjan Patil, M.B.B.S, MD Microbiology, Dept. of Microbiology and Molecular Biology 

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HCV-Hepatitis C RNA Detection by Taqman Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the HCV-Hepatitis C RNA Detection by Taqman with a clear pricing structure.

The HCV-Hepatitis C RNA Detection by Taqman Price in Mumbai is ₹ 4,660 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

HCV RNA test is often done as a reflex to positive HCV antibody detection.
Test can detect HCV infection before the appearance of HCV antibodies
It can also be used to confirm presence of chronic (active) HCV infection 

This test is often done as:

  • a reflex to positive HCV antibody detection.
  • detect HCV infection before the appearance of HCV antibodies
  • used to confirm the presence of chronic (active) HCV infection
  • can detect the amount of HCV present in the blood known as viral load
  • used to monitor the viral load during and after the treatment
  • used to confirm whether one has a high or low viral load
  • used to determine if liver biopsy is further required
  • can be used to develop a comprehensive treatment plan
  • monitor the progress of the treatment
  • can determine if specialist treatment is required in addition

Presence of HCV RNA in serum

The HCV RNA Detection by TaqMan RT PCR test measures the presence of HCV RNA in serum or plasma.

HCV RNA test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer. 

A "Positive" HCV – RNA result indicates HCV infection in the individual

A "positive" HCV – RNA result indicates HCV infection in the individual. On the other hand, a "negative" HCV - RNA result shows the absence of the Hepatitis C virus in the individual

It is suggested that all adults, pregnant women and people with risk factors for Hepatitis C virus should get the test done.

People with the following symptoms should do the HCV RNA Detection by TaqMan RT PCR test:

  • 18 years or older
  • Pregnant (must be tested during each pregnancy
  • if drugs are being injected, regular testing is recommended
  • Injected drugs even once, many years ago
  • HIV-AIDS positive individuals
  • Liver profile shows an abnormality
  • Having any liver disease
  • People who are put on haemodialysis
  • received organ or blood donation before July 1992
  • Received clotting factor concentrates before 1987
  • Have been exposed to blood from a person who has hepatitis C
  • Were born to a mother with hepatitis C

If the above risk factors are present, in addition to certain signs like yellowing skin or eyes, loss of appetite, stomach upset, nausea or vomiting sensation, pain in the stomach, fever, urine which is dark in colour, light-coloured stool, pain in the joints and tiredness, one is suggested to get tested for Hepatitis C virus which may be present in the blood.


  1. Khalvati Fahlyani, B., Behzad-Behbahani, A., Taghavi, S. A., Farhadi, A., Salehi, S., Adibzadeh, S., Aboualizadeh, F., Alavi, P., Nikouyan, N., Okhovat, M. A., Ranjbaran, R., Rafiei Dehbidi, G. R., & Shakibzadeh, A. (2015). Development of an In-House TaqMan Real-Time RT-PCR Assay to Quantify Hepatitis C Virus RNA in Serum and Peripheral Blood Mononuclear Cells in Patients With Chronic Hepatitis C Virus Infection. Hepatitis monthly, 15(8), e28895.

It is ideal to get tested if one has symptoms, such as yellow skin or eyes, not wanting to eat, upset stomach, throwing up, stomach pain, fever, dark urine, light-coloured stool, joint pain, and feeling tired

There are no specific preparations required for this test. Inform the doctor of any medical conditions, drugs taken or any allergies such that precautions can be taken accordingly.

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