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FSH LH TSH Prolactin Profile Test

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FSH LH TSH Prolactin Test Overview

FSH LH TSH Prolactin test is a blood test to measure hormonal levels of FSH (Follicle stimulating hormone), LH (Luteinizing hormone), Prolactin and TSH in blood. All these four hormones are produced by the pituitary gland. 
FSH and LH are present both in men and women. In women it controls the menstrual cycle and stimulates the production of eggs (ovulation). In men it stimulates the production of sperm. It is present in low levels in children until puberty, post which the FSH levels increase to stimulate the production of oestrogen and testosterone.
Prolactin acts primarily on the mammary glands and its principal action involve - proliferation of mammary glands ; initiation of milk secretion . The levels of this hormone rises in pregnancy and during lactation. 
TSH is synthesized by the pituitary gland and promotes the stimulation of T3 and T4 hormones and controls the metabolic rate in body


The FSH-LH-Prolactin-TSH test is used to measure the amounts of certain hormones in the blood. FSH stands for Follicle Stimulating Hormone, LH for Luteinising Hormone and TSH for Thyroid Stimulating Hormone. FSH in women is especially active during the first two weeks of the period cycle as it makes the ovaries produce increased amounts of estrogen and some progesterone. FSH maintains normal production of sperm in men. LH has similar roles in the fertility function in both men and women. TSH helps in the production of thyroid hormone which maintains normal metabolic functions of the body.

All of these hormones are secreted by the pituitary gland in the brain. The hormones FSH and LH are present in both men and women. In women, it helps in maintaining the menstrual cycle and plays a role in ovulation. In men, FSH helps in maintaining normal sperm production. These hormones are present in very small amounts in children till they hit puberty.

Prolactin is present in women and it stimulates milk production from the mammary glands. Prolactin increases especially during pregnancy and lactation.

TSH stimulates the thyroid gland to produce hormones T3 and T4 and it plays a role in controlling the metabolism of the body.

Written by: Dr.Shibani R, Medical Writer, Medical Affairs

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FSH LH TSH Prolactin Profile Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the FSH LH TSH Prolactin Profile Test with a clear pricing structure.

The FSH LH TSH Prolactin Profile Test Price in Mumbai is ₹ 1,665 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

FSH-LH-Prolactin-TSH test is done to diagnose fertility issues in males and females. In women, the reason depends on at what age and stage it is done. It may be done to diagnose menstrual irregularities, or any dysfunctions . In women having difficulties conceiving, it is done to diagnose the cause for infertility. It may also be done to diagnose any thyroid disorders which may affect the reproductive health. It is done in males to diagnose reasons for infertility, low sperm count, testicular issues or any thyroid disorders. In children it may be done to diagnose early or delayed puberty based on the levels.

This test is used to check for fertility issues among women as well as men. If facing irregularities in menstrual cycles, this test can help find out the reasons behind it. In women of reproductive age, this test is used to evaluate the reasons behind infertility.

In males, it is chiefly done to check for infertility, reduced sperm count, testicular issues or thyroid disorders. In children, these tests are used to diagnose delayed or early puberty.

If you have the following issues, your doctor will recommend this test:

  • Difficulty conceiving
  • Infertility in men and women
  • Low sperm count
  • Irregular periods/PCOS/PCOD
  • Sudden weight gain
  • Early puberty
  • Late puberty
  • Difficulty in lactation after delivery
  • Slow or high metabolism

FSH lh tsh prolactin test measures the levels of FSH, LH, Prolactin and Testosterone hormones in the blood.

During fsh lh tsh prolactin test blood sample is taken from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. You may feel a  little sting when the needle goes in or out. This usually takes less than five minutes. In new-borns the blood is taken from the skin behind the heel.

FSH-LH-Prolactin-TSH levels are important for successful conception. Abnormal levels in any of these may lead infertility issues. 
High FSH-LH-Prolactin-TSH levels in women may mean primary ovarian insufficiency or polycystic ovarian syndrome (PCOS), ovarian and uterine malignancies. High TSH may cause hyperthyroidism affecting the sexual function and reproduction. In men, high FSH-LH-Prolactin-TSH  may indicate testicular issues, sexual dysfunctions or low sperm count. All these may indicate infertility in men. In children, high levels before puberty, signify early onset puberty. 
Low levels may lead to lack of ovulation and sperm production leading to infertility issues in both men and women. Low levels may be due to problems in the hypothalamus or pituitary gland, being underweight or other central nervous system issues. Low TSH levels lead to hypothyroidism in males and females.

Wear loose-fitting clothes. Ask the doctor about any doubts one may have. Drink only water for 8-12 hours before the test. The FSH and LH are best taken on the 2nd or 3rd day of the menstrual cycle.

The reference range for FSH is as follows:


Follicular phase: 3.1-7.9 mIU/mL

Ovulation peak: 2.3-18.5 mIU/mL

Luteal phase: 1.4-5.5 mIU/mL

Postmenopausal: 30.6-106.3 mIU/mL


Age 0-7 years: <6.7 mIU/mL

Age 8 years-adult:1.3-19.3 mIU/mL

The following values are considered to be normal LH blood levels:


Follicular phase of the menstrual cycle: 1.9 to 12.5 IU/L

LH surge: 8.7 to 76.3 IU/L

Luteal phase of the menstrual cycle: 0.5 to 16.9 IU/L

Pregnancy: less than 1.5 IU/L

Post-menopause: 15.9 to 54.0 IU/L

Women using contraceptives: 0.7 to 5.6 IU/L


Age group of 20 and 70 years: 0.7 to 7.9 IU/L

>70 years of age: 3.1 to 34.0 IU/L

The following values are considered to be normal prolactin blood levels:

Males > 18 years: 2.10 - 17.70 ng/mL

Adult Females

Non-pregnant: 2.80 - 29.20 ng/mL

Pregnant: 9.70 - 208.50 ng/mL

Post Menopausal: 1.80 - 20.30 ng/mL

The normal values for TSH are as follows:

Men and Women:

>/= 20 years: 0.40 - 4.50 mIU/L

Pregnant Women:

First Trimester: 0.26-2.66 mIU/L

Second Trimester: 0.55-2.73 mIU/L

Third Trimester: 0.43-2.91 mIU/L

Some of the tests that are ordered along with the FSH-LH-Prolactin-TSH test:

  • Complete Blood Count (CBC)
  • Anti-Mullerian Hormone (AMH) test
  • Insulin (fasting) test
  • Hb1Ac, Glycated Hemoglobin test


  1. Wheeler M. J. (2013). The measurement of LH, FSH, and prolactin. Methods in molecular biology (Clifton, N.J.), 1065, 105–116.
  • ·       Hormone Profile
  • Fertility Profile
  • ·       Follicle Stimulating Hormone (FSH)
  • ·       Luteinising Hormone (LH)
  • ·       Prolactin (PRL)
  • Thyroid Stimulating Hormone (TSH)
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