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AFB MDR Screen Hain's Line Probe Assay Extra pulmonary samples

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MTB Test Overview

Tuberculosis is a chronic respiratory tract infection causing fever, cough and weight loss. It is commonly caused by Mycobacterium tuberculosis (M.tb). Many diagnostic techniques are available for diagnosis of tuberculosis which include staining, culture and nucleic acid detection. Among nucleic acid detection techniques are polymerase chain reaction (PCR), Cartridge based nucleic acid amplification technique (CBNAAT), Line probe assay (LPA), Next generation sequencing (NGS).
LPA is based on DNA strip technology. This test may be used to detect M.tb and resistance towards rifampicin and isoniazid resistance in culture isolates.

Extrapulmonary tuberculosis (EPTB) diagnosis is difficult. This is due to its unusual clinical presentation, It usually causes a delay in or denial of therapy. Along with quick case discovery, it is critical in these circumstances to determine the MDR status early.

The Mycobacterium tuberculosis (MTB) complex and INH and Rifampicin resistance are both detected using the commonly available GenoType MTBDRplus assay version 2.0 (Hain Lifescience, Nehren, Germany).

Doctors hope to assess the role it plays in the diagnosis and detection of resistance. They do this by comparing this test's performance to the gold standard, which is culture. They also compare it to the composite reference standards (CRS) in the identification of EPTB. On negative and positive direct smear specimens, it generally had dubious benefits.

In cases when MDR-TB is highly suspect, experts recommend the Hain test. This is to identify the infection. They delay using traditional identification results to verify NTM. A positive Hain test results in a negative direct smear.

Test Overview

Tuberculosis is a chronic respiratory illness. It results in,

  • Fever.
  • Cough.
  • Loss of weight.

Usually, Mycobacterium tuberculosis (M.Tb) is to blame for these symptoms and the disease. For the identification of tuberculosis, there are numerous diagnostic methods available -

  • Staining.
  • Culture.
  • Nucleic acid detection.

Nucleic acid detection can be done using any one of the following methods -

  • PCR or polymerase chain reaction.
  • CBNAAT or cartridge-based nucleic acid amplification technique.
  • LPA or line probe assay.
  • NGS or next-generation sequencing

The DNA strip technique underlies LPA. This test may be used to identify M. TB in culture isolates as well as rifampicin and isoniazid resistance.

Who should do the AFB MDR Screen by Hains Line Probe Assay - Extra Pulmonary Samples test?

If you experience any of the potential symptoms of TB like unexplained coughing or fever that lasts for weeks, you should get tested.

What Other Tests Are Ordered Along with the AFB MDR Screen by Hains Line Probe Assay - Extra Pulmonary Samples test?

Mycobacterium tuberculosis Primary Panel

Mycobacterium tuberculosis Secondary Panel

Mycobacterium avium-intracellular Complex

Written by: Dr Vishal Wadhwa, M.D, D.N.B Microbiology, Medical Affairs

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AFB MDR Screen Hain's Line Probe Assay Extra pulmonary samples Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the AFB MDR Screen Hain's Line Probe Assay Extra pulmonary samples with a clear pricing structure.

The AFB MDR Screen Hain's Line Probe Assay Extra pulmonary samples Price in Mumbai is ₹ 2,000 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

MTB test is often used as a follow up test to Xpert Mtb/Rif results to confirm resistance to Rifampicin and detect resistance against Isoniazid.
It can detect M.tb and resistance towards Isoniazid and Rifampicin

MTB test detects DNA of Mtb and mutations which can lead to Rifampicin and Isoniazid resistance

In extrapulmonary samples, the GenoType MTBDRplus assay can provide an early diagnosis. It can also provide high sensitivity for both INH and Rifampin.

Laboratory will require culture isolate to book this test. For further details pls contact customer care

M.tb detected - This means that the DNA of M.tb is present in sample and patient is likely to be suffering from tuberculosis.
M.tb Not detected - This means that the DNA of M.tb is absent in sample and patient is unlikely to suffer from tuberculosis.  
Resistance (mutations) to Rifampicin, Isoniazid (KatG), InH A promoter region are reported by the test

This test utilizes a pure culture made from bodily fluids such as,

  • Sputum.
  • Bronchial alveolar lavage.
  • Cerebrospinal fluid (CSF).
  • Pus.
  • Tissue.
  • biopsies.
  • lymph nodes.
  • abscess.
  • urine.

Before taking this test, no extra preparation is necessary.

For this test to be scheduled, the laboratory needs a culture isolate. Please get in touch with customer service for more information.

  • Amikacin: 6 µg/mL.
  • capreomycin: 10 µg/mL.
  • cycloserine: 60 µg/mL.
  • ethionamide: 10 µg/mL.
  • kanamycin: 6 µg/mL.
  • PAS: 8 µg/mL.
  • streptomycin at a low level (2.0 µg/mL) and a high level (4.0 µg/mL).
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