AFB DRUG SUSCEPTIBILITY PANEL 14 DRUGS

This test is done when drug resistant is suspected in a case of tuberculosis (for e.g., contact with a drug resistant/ failed treatment case, failure of 1st line treatment, previous history of use of 2nd line treatment drugs), or the resistance has been detected by nucleic acid based tests.
Results of susceptibility testing by MGIT are gold standard and take up to 4 weeks but are still recommended by WHO to confirm findings by LPA & NGS

When it is thought that a tuberculosis case may be drug-resistant, this test is performed. The likelihood may rise in the following circumstances:

1. Contact a patient who has failed therapy or is drug-resistant.
2. First-line treatment's failure.
3. Use of medications in second-line therapy in the past.

Nucleic acid-based techniques have been used to identify the strain. The results of MGIT's gold standard susceptibility testing are still advised by WHO. It may take up to four weeks to confirm the results.

This test detects resistance of pure culture of M. tb towards the first line of drugs i.e., Isoniazid, Rifampicin, Ethambutol and pyrazinamide

Tuberculosis is a chronic respiratory tract infection. It causes high fever, weight loss, and cough. It is caused by the bacterium Mycobacterium tuberculosis (M.tb). Several diagnostic methods are available for tuberculosis diagnosis. These include culture, staining, and nucleic acid detection. Among nucleic acid detection techniques are,

1. Polymerase chain reaction (PCR).
2. Cartridge-based nucleic acid amplification technique (CBNAAT).
3. Line probe assay (LPA).
4. Next-generation sequencing (NGS).

This test offers sensitivity testing for fourteen antitubercular agents -

1. Isoniazid.
2. Rifampicin.
3. Ethambutol.
4. Pyrazinamide.
5. Streptomycin.
6. Kanamycin.
7. Moxifloxacin.
8. Amikacin.
9. Ethionamide.
10. Clofazimine.
11. Capreomycin.
12. Bedaquiline.
13. Linezolid.
14. Levofloxacin.

Fresh patient sample is not required (except when requested)

The results of the testing will be given for each antibiotic separately. Depending upon the resistance detected doctor will prescribe the anti-tubercular treatment. The following fourteen antitubercular agents are tested: Isoniazid, Rifampicin, Ethambutol, Pyrazinamide, Streptomycin, Kanamycin, Moxifloxacin, Amikacin, Ethionamide, Clofazimine, Capreomycin, Bedaquiline, Linezolid, Levofloxacin.
Sensitive - means that bacterium will respond the antibiotic tested.
Resistant - means that bacterium will not respond to the antibiotic tested.
 

There is no preparation needed to get ready for the test. You have to only rinse your mouth thoroughly with water before taking a sputum sample. If there is anything else, your doctor will guide you.

There is no special preparation required for this blood test. A fresh patient sample is not required (except when requested).

1. Ethambutol: 5.0 µg/mL.
2. Isoniazid: 0.1 µg/mL (0.4 µg/mL if resistant to 0.1 µg/mL).
3. Pyrazinamide: 100 µg/mL.
4. Rifampin: 1.0 µg/mL.

Antimicrobial Susceptibility Test

AFB Susceptibility Panel

AFB Antibiotic Susceptibility test