Metropolis Laboratories offers in excess of 4,500 tests and test combinations, ranging from routine screening tests to super specialised molecular and genetic assays. Metropolis has always been in the forefront in employing latest technology and automations, adopting stringent internal and external quality control mechanisms and making planned investments in research and development.
Metropolis is India’s first multi-national chain of diagnostic centres with 105 state of the art laboratories in 7 countries and over 700 collection centres across countries. Our Laboratory Services has been segmented in to 7 core functions. Though many tests and test combinations overlap with each other, all section units work in tandem. The segmentation is critical since the techniques, automation and quality process that each section demands in unique from each other.
Clinical chemistry is the area of clinical pathology that is generally concerned with analysis of blood (Whole blood, serum, plasma), urine, body fluids for screening, diagnosis and monitoring of many diseases.
When is it used?
Tests done in clinical chemistry are varied and largely used for cardiovascular disorders, endocrine disorders like diabetes, thyroids, kidney disorders, cancers, infertility, pregnancy, nutritional disorders (Vitamins, heavy and trace metals), new born screening, autoimmunity and allergy, infections, etc.Tests are designed to detect these changes qualitatively or quantitatively and compared to the biological reference Interval of human beings.
Clinical pathologists and biochemists use a wide range of techniques including Immunoassays like ELISA, chemiluminescence, enzyme linked flourescence, radioimmunoassay; Biochemical methods like colorimetry, enzymatic reactions, spectrophotometry, nephelometry, electrophoresis; Analytical chemistry methods like high performance liquid chromatography, atomic absorption, mass spectrometry, infrared spectrometry, etc.Technicians and experts at Metropolis have developed complex practical and interpretive skills for manual techniques and management of highly automated testing systems. All assays are closely monitored and quality controlled.
We are equipped with the latest US FDA approved automations and infrastructure, CE marked reagents, updated with advanced and newest technologies and armed with a team of experts who bring to the table a world of experience.
Clinical Microbiology is one of the most evolving branches of laboratory medicine. The clinical microbiology laboratory is central to the diagnosis and management of infectious diseases. Accurate and timely infectious disease diagnosis is crucial in appropriate therapeutic management of patients. In the current era of multidrug resistance, clinical microbiology laboratories are crusaders in antibiotic stewardship by providing antimicrobial resistance data to physicians and hospitals.
Clinical Microbiology testing encompasses a broad range of infectious disease testing in major broad areas such as Bacteriology, Mycobacteriology, Mycology, Parasitology, Infectious Immunology (Serology) and Molecular Microbiology.
A range of techniques are employed in the laboratory to process specimens and ensure accurate and timely results. Use of state of the art techniques and technologies has a positive impact on the workflow and quality of results.
Special stains such as Modified Kinyoun, Trichrome, LPCB, AuramineRhodamine, etc are used for morphological identification of bacteria, parasites and a variety of fungi.
A variety of enriched, differential, chromogenic and selective media are used at various incubation temperatures and atmospheric conditions (aerobic, anaerobic, capnophilic and microaerophilic) to ensure growth of common as well as fastidious pathogens.
Microbroth dilution techniques, KirbyBauer disc diffusion techniques, biochemical analysis are employed effectively to tackle with the diverse range of pathogens encountered.
BACTEC FX: Automated continuous monitoring system for blood cultures for quicker interventions in Sepsis.
Vitek2 Compact: Automated efficient system for faster accurate identification of pathogens. Complemented by an Advanced Expert system for susceptibility testing of pathogens, Microbrothdilution (MIC) testing is offered to clinicians for targeted therapy of patients.
BACTEC MGIT 960: The newer Gold standard in Tuberculosis is a fully automated liquid culture technology for rapid culture of Mycobacteria. The same system is also designed to carry out susceptibility testing for the complete panel of anti-TB drugs.
Sysmex UF-1000i: Automated urine sedimentanalyzer system for Flowcytometry based morphology analysis of urine specimens.
Immunology Services:A range of platforms employing ELISA, CLIA, CMIA, ELFA and IF(Immunofluorescence) techniques for antigen antibody detection.
Molecular Microbiology: Fully automated latest molecular platforms such as Qiasymphony,Roche COBAS® AmpliPrep/COBAS TaqMan ,Cepheid's GeneXpert, Applied Biosystems® 3500 Dx Genetic Analyzer,Rotor-Gene® Q MDx 5plex HRM Platform, are used to offer faster, sensitive diagnosis of viral, bacterial and fungal agents. Apart from qualitative and quantitative testing, we offer Genotyping (HIV, HBVand HCV) and Drug Resistance testing for (HBV, HIV).
Clinical Microbiology is a science of interpretive judgement. When dealing with complex pathogens; we offer Expert consultations on diagnostic report interpretations which will assist in therapeutic decision making.
What sets us apart is our expertise and experience built over the years and our relentless commitment to quality supported by our highly trained technical team.
The state of art Metropolis Microbiology laboratory is one of few laboratories offering a complete range of infectious disease referral services.
One of the few laboratories in the country to offer anaerobic and comprehensive parasitology testing.
One of the few laboratories in the country to offer organism-isolate specific panels as per latest CLSI guidelines. Our capabilities include guidance on antibiotic resistance monitoring and infection control practices.
Metropolis Mycobacteriology laboratory is RNTCP accredited and an active member of IPAQT initiative for Tuberculosis.
Apart from liquid culture (MGIT) and Susceptibility for M. tuberculosis, the laboratory also offers a complete range of Molecular drug resistance testing services including Genexpert, Line probe assays. Our Algorithm based screening and innovative panels in Tuberculosis incorporating multiple levels of testing from Microscopy to Culture to Molecular play a vital role in ensuring accurate diagnosis.
Also, we are one the few laboratories in the country to offer molecular identification of Nontuberculous Mycobacteria (NTM) and their susceptibility testing.
Mycology capabilities include morphological identification of a variety of Candida and fungi including Dermatophytes. We also offer automated susceptibility and MIC testing for yeasts. Comprehensive capabilities, guidance on appropriate specimen collection, extensive support are vital when treating complex or chronic infections. Our innovative test menu and expert skills would help you to tackle a range of resistant, newly emerging or remerging strains and pathogens.
Cytogenetics is a branch of genetics that is concerned with the study of the structure and function of the cell, especially the chromosomes in health and disease.
When is it used?
Genetic studies are an important laboratory diagnostic procedure in
Genetic Counseling services: Metropolis is the only lab in India to offer Genetic Counselling services to its customers. It is an education and training initiative which aims to spread knowledge about genetic aspects of illness in terms of risk analysis, presence of heritable disorder or of chances of passing it to their unborn offspring with following principle elements: diagnostic and clinical aspects, documentation of family and pedigree information, recognition of inheritance patterns risk estimation, scientific communication and empathy in those seen, information on available options and further measures, support in decision making and discussion on cost effectiveness.
Genetic counselling can be offered to large number of genetic disorders for different conditions and different age groups.
Tissues used for analysis: Blood, Bone Marrow, Amniotic fluid, CVS, Cord blood, Abortus material (POC – products of conception)
It includes routine analysis of G-banded chromosomes, other cytogenetic banding and staining techniques, as well as molecular cytogenetics such as Fluorescent in situ Hybridization (FISH) and comparative genome hybridization. New techniques allow for increased resolution of chromosome banding patterns, permitting differentiation of a greater number of abnormalities.
Our trained team of specialists combined with our 33 year experience ensure accurate clinical correlation. In addition to our NABL, CAP Accreditation and adherence to US FDA approved standards, our Centre is also authorized under the PCPNDT Act for prenatal testing. We offer expert opinion services in the form of interpretative comments, clinical correlation and report discussion.
Fully accredited laboratory, wide range of Genetic services and counselling services, well qualified, experienced and dedicated team of Medical Doctor, Senior Scientists, and technologists. Competitive and reasonable charges, minimum turn-around time, priority to paediatric and critical cases, and all results are interpreted by Qualified Medical Geneticist.
Highly qualified and experienced clinical Geneticist available for clinical correlation, interpretation of report, and Genetic Counseling.
Cytogenetics (Conventional Karyotyping, High Resolution Banding, Breakage studies, staining and Banding techniques)
Molecular Cytogenetics (FISH) for Aneuploidies on various tissues including sperm FISH,various microdeletions, Solid tumors and hematological malignancies.
PCR/Sequencing based Molecular studies: Thalassemia, DMD/BMD, SMA, SCA, Fridreich Ataxia, Huntigton Disease Y-microdeletion, Fragile X, Paternity test, Factor II and Factor V.
Techniques & Automation:
A team of Geneticists and senior scientists available on telephone for any queries right from sample selection to report discussion.
Highly qualified and experienced Clinical Geneticist available for clinical correlation, interpretation of report, and Genetic Counselling.
Haematopathology & Hemostasis are the branches of pathology which studies diseases of hematopoietic (blood) cells & blood components. The Hematopathology laboratory provides diagnostic evaluation of blood, bone marrow, and related biological samples for diagnosis of specific haematological diseases including Anemias, Leukemias/Lymphomas, Thalessemias, Haemoglobinopathies, etc.
Haematopathology at Metropolis is a large referral tertiary care unit comprising various routine & ancillary specialities like Flow cytometry, Abnormal Haemoglobin studies by HPLC, Transfusion medicine by CAT (Column Agglutination Technique), Fully automated 5-part cell counter and coagulation systems.
Metropolis is one of the very few laboratories to offer round the clock pathologist review. We also offer aggressive TAT (Turn-around Time) for critical tests like Malarial Parasite, Platelet Count, Prothrombin Time etc. With a 33 year experience in delivering accurate and reliable results, we are one of the trusted partners to clinicians and hospitals alike.
Metropolis is the only laboratory which gives complete diagnostic workup of Thalessemias & Haemoglobinopathies by testing on alternate platform (Capillary Electrophoresis) & Molecular methods like DNA PCR & DNA Sequencing, apart from HPLC.
Metropolis offers the comprehensive packages for complete workup of Leukemias & Lymphomas by performing reflex tests by Immunophenotyping, Cytogenetics (FISH) & Molecular methods, post morphology assessment.
Metropolis offers the Blood group test by automated CAT (Column Agglutination Technique) method which is more sensitive & specific to routine slide & tube technique methods.
Techniques & Automation:
Electrophoresis is a method of separation of protein compounds in a salt solution based on their electrical charges when placed in an electric field. The varieties available are Agarose gel Electophoresis, Cellulose gel Electophoresis, Capillary Electophoresis. This technology is used in clinical field for serum Protien Electophoresis, urine Protien Electophoresis, serum Immunofixation, estimation of various isoenzymes (CPK, LDH, Alkaline Phosphatase), CSF Oligoclonal Bands.
Column Agglutination Technique:
This is a gel card based technique where the gel column is used to separate out the agglutinated RBCs from non-agglutinated ones. The investigations done on this technique are Blood Group, Rh antibody titre, Indirect & Direct Coobs test. This method has advantage over conventional slide & tube technique in having higher sensitivity & specificity
High Performance Liquid Chromatography (HPLC):
High performance liquid chromatography (HPLC) is a form of liquid chromatography to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by injecting the sample mixture onto the column where the different components in the mixture pass through the column at different rates due to differences in their affinity and charges in the mobile liquid phase to the stationary phase. These components elutes out through the column at different retention times unique for the components, which can be quantified. The clinical application of this technology is for Haemoglobinopathies &Thalassemias, estimation of Vitamins, Glycated haemoglobin, Hormones, etc.
Flow cytometry is a unique and novel technology to assess the characteristics of the cells from biological cellular samples which help in arriving at specific medical diagnosis. It functions based on fluidis, optics & electronic mechanisms,allowing simultaneous multiparametric analysis of the physical and/or chemical characteristics more than thousands of cells in very short time span. The clinical application of flow cytometry has emerged from analysis of T-cell subsets (CD3/4/8/19/NK cells) in immunodeficiency syndromes, immunophenotyping in hematolymphoid malignancies to analysis of platelets to enumeration of stem cell population in bone marrow transplantation, DNA content and cell cycle parameters in a wide range of malignancies & molecular microbiology.
This technique is used to differentiate the various white blood cells in blood for automated differential counts in a CBC report. The cell differentiation occurs based on unique characteristics of cell related to volume (V), conductance (C) & scatter (S). This system analyses approximately 8000 cells for giving differential count as compared to 100 or 500 manual differential counting, giving accurate & precise results.
A fully automated coagulation tests system for all the routine and specialised assays. It encompasses three different methodologies like optical, chromogenic & immunoturbidometry required for varied testing parameters.
Molecular diagnostics is a dynamic and transformative area of diagnostics, leading to insights in research and treatment in many disease states that are revolutionizing health care. Molecular diagnostics detect and measure the presence of genetic material or proteins associated with a specific health condition or disease, helping to uncover the underlying mechanisms of disease and enabling clinicians to tailor care at an individual level – facilitating the practice of “personalized medicine.”
Why is it used?
“Molecular diagnostics” is a broad term describing a class of diagnostic tests that assess a person’s health literally at a molecular level, detecting and measuring specific genetic sequences in deoxyribonucleic acid (DNA) or ribonucleic acid (RNA).
The molecular diagnostics industry is growing rapidly, thanks to the inherent accuracy, sensitivity, specificity, and quick turnaround of molecular techniques. In applying molecular diagnostic technology, different methods can be utilized to address different questions.
Metropolis is a leader in molecular diagnostic testing and offers comprehensive test menu through more than 200 molecular diagnostic tests. These tests fall under different areas of medicine, including infectious disease, oncology, genetic testing, human leukocyte antigen typing (which investigates and predicts immune function), predictive diagnostics (identification of a disorder before appearance of clinical symptoms), and pharmacogenomics—the genetic prediction of which drugs will work best. In addition, we offer pre- and post-test counseling.
Surgical pathology is the most significant and time-consuming area of practice for most anatomical pathologists. It includes Histopathology, Cytopathology & Immunohistochemistry.
Histopathology: It is the study of tissues removed from living patients during surgery to help diagnose a disease & determine a treatment. It includes both the physical examination of the tissue with naked eye as well as examining processed tissue under microscope.
Cytopathology: It is examination of individual cells & small clusters of cells, may be used for the diagnosis & screening of disease, including cancers. It includes Gynaec and Non-gynaec cytology.
Immunohistochemistry: It is the process of detecting antigen in cells of a tissue by exploiting the principle of antibodies specific to antigen in their biologic genome. It is a powerful tool with overlapping features among diagnosis, theranostic and genomic applications.
The three major units at Metropolis are Histopathology, Cytopathology and Immunohistochemistry. Metropolis offers a comprehensive Histopathology test menu including 21 special stains. Cytopathology test menu includes routine, cytospin & liquid base technical preparation. In Immunohistochemistry along with automated IHC there are more than 100 IHC markers, 5 panels of multiple IHC cocktails, and CISH for EBV, HPV& CMV.
Why Metropolis is a class apart
Our Histpathology Department processes samples annually across India and abroad. We offer the most exhaustive NABL accredited Directory of services with over 15 special stains & 110 IHC markers. We are the first lab in India to establish Liquid Based Cervical Screening Co-test including Cytology & HPV with reflex to CISH “Molecular PAP”. We offer Multiplex IHC – 5 panels covering Lymphoma, Prostate carcinoma, Lung Carcinoma & Myeloma, We also offer Chromogenic In – Situ Hybridization (CISH) for EBV, HPV & CMV.
We have again been in the forefront in offering Automated IHC Staining. We also offer comprehensive & complete Oncology reports with Molecular tests such as Her 2 neu by FISH, EGFR mutation, K rasmutation , HPV and CISH & ALK 1 by FISH.
To top it all up, we have the best turn around time and excellent customer care support with minimal response time for technical & case related queries.
Regular CMEs, workshops, training programmes are organized in India & abroad.
We have pioneered the concept of SUBSPECIALITY EXPERT OPINION – for Carcdiovascular, Lung, Transplant, Gastrointestinal ,Hepatobiliary, Dermatopathology, Hematolymphoid , Veterinary & Breast Pathology. We are also the very few laboratories in India to offer Automated FDA approved Liquid Based Cytology for Gynaecologic & Non-Gynaecologic specimens and we already have about a half decade experience in this area.
In House Metropolis Experts:
|Dr. Anuradha Murthy||MD, (Path) DPB||Senior Consultant Surgical Pathologist|
|Dr. Kirti Chadha||MD, PDCC (Oncopath)||Consultant Surgical Pathologist|
|Dr. VikasKavishwar||MD, DNB, DPB||Consultant Surgical Pathologist|
|Dr. Kush Raut||MD (Path)||Surgical Pathologist|
|Dr. S.M. Barodawala||MD (Path)||Surgical Pathologist|
|Dr. Kunjal Sheth||MD (Path)||Surgical Pathologist|
Call 022-30840772 to get in touch with in-house experts
How can I get my patients' results?
Reports can be delivered via fax, mail, courier, or via the Internet with our secure web-based application that allows physicians 24/7 access to laboratory reports.
Do you offer a slide consultation service for second opinion?
Yes. Our specialists offer consultative services that provide guidance for additional diagnostic workup, prognostic information, pathogenetic insight, and therapeutic direction. Please forward the slides, previous surgical pathology reports and, if possible, the paraffin blocks, along with the request.
Do you offer slide preparation and technical services?
Yes, Metropolis Laboratories provides histologic preparation and immunohistochemistry in addition to comprehensive diagnostic services.
For more queries on any assistance on samples and reports, please call: Ms. Ashwini Patkar 022- 30840791
Translational research is the basis for the testing performed at Metropolis Laboratories. We are committed to the adoption of the latest technologies in order to provide the highest quality and most cost-effective testing services to meet clinical needs. A reference lab needs have an exhaustive test menu and it is important to add new tests whenever there is a demand due to increased awareness. Some of these tests give an added value to the organization and an edge over the peer labs.
The R&D division plays a major role in fulfilling these ever growing demands by evaluating and coordinating the overall developmental activities related to new tests and technologies. We have the expertise to create, implement and validate clinical tests across a wide range of technologies, including PCR, Real Time PCR, and DNA Sequencing etc.
Our expertise includes:
The new test development procedure involves thorough literature search, technology evaluation, establishing a reference range if required and preparation of flyers and brochures to educate the doctors.
Two key features of the test developed at Metropolis are use of latest technology and cost effectiveness.
The team closely monitors the recent developments in the field of diagnostics and takes the charge of developing and eventually offering them to the patients.
R&D also plays a role in imparting the domain knowledge to the biotechnology students through training programs and projects.